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題 名 | 喜樹癒合組織之培養與喜樹鹼含量分析=Callus Culture and Camptothecin Production of Camptotheca Acuminata |
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作 者 | 張淑華; 蔡錦瑩; 何政坤; 黃芷雲; | 書刊名 | 臺灣林業科學 |
卷 期 | 21:4 民95.12 |
頁 次 | 頁513-521 |
分類號 | 436.184 |
關鍵詞 | 喜樹; 癒合組織; 喜樹鹼; Camptotheca acuminata; Callus culture; Camptothecin; |
語 文 | 中文(Chinese) |
中文摘要 | 以喜樹下胚軸與2個月大試管種子苗之葉片,建立癒合組織誘導與培養方法,結果兩者癒合組織生長最佳的培養基均為WPM基本培養基添加1~3 mg L-1 NAA或2,4-D。唯NAA所誘導的癒合組織較硬,而2,4-D誘導的癒合組織較為鬆軟。分析19種來自下胚軸、葉片、莖段之3個月大癒合組織的喜樹鹼 (CPT) 與10-烴基喜樹鹼 (HCPT),發現CPT含量與材料來源無關,而HCPT只存在於莖段癒合組織,兩個化合物在不同細胞系間差異很大,CPT含量最高為0.0307% (細胞乾重),HCPT最高為0.0021%,最低則都趨近於0。調查6個經3年長期培養於NAA與2,4-D的癒合組織之喜樹鹼含量,發現只有1個培養於NAA的癒合組織,在培養的3年裡,可穩定維持生產喜樹鹼,另外有4個癒合組織的喜樹鹼含量會逐漸下降,尤其是培養於2,4-D之癒合組織幾乎完全喪失了生產喜樹鹼的能力,本研究結果顯示植物生長激素的選擇與細胞株的篩選,對利用細胞培養生產喜樹鹼而言,非常重要。 |
英文摘要 | Callus cultures of Camptotheca acuminata Decne. were initiated from hypocotyl and leaf explants excised from 2-mo-old plants in vitro. The optimal culture medium for callus growth for both explants was woody plant medium (WPM) containing 1~3 mg L-1 NAA or 2,4-D. The calli appeared compact in NAA medium, but friable in 2,4-D medium. Comparing HCPT (10-hydroxy camptothecin) and CPT (camptothecin) contents of calli derived from 19 sources including the hypocotyls, leaves, and stems of different donor plants, we found that CPT existed in all 3 organ-derived calli but HCPT was present only in stem-derived calli. Both CPT and HCPT contents in different callus lines varied greatly. CPT and HCPT contents in a superior line were 0.0307% and 0.0021%, respectively, while those of the poor lines were close to 0. Investigating the stability of CPT content in long-term cultures, 6 calli subcultured monthly in NAA and 2,4-D media for over 3 yr were analyzed once every 6 mo. Only 1 callus line cultured in NAA medium maintained a stable CPT content of between 0.01% and 0.014%. Four lines gradually lost the ability to produce CPT, especially those cultured in 2,4-D medium. This indicates that the type of auxin used and the callus line are very critical for CPT production via cell culture. |
本系統中英文摘要資訊取自各篇刊載內容。