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題 名 | 金草蘭微體繁殖技術之研究=Study on Micropropagation of Dendrobium Clavatum Lindl. |
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作 者 | 李淑真; 廖芳心; 鄭隨和; | 書刊名 | 桃園區農業改良場研究彙報 |
卷 期 | 56 民93.12 |
頁 次 | 頁18-25 |
分類號 | 435.431 |
關鍵詞 | 金草蘭; 微體繁殖; Dendrobium clavatum Lindl.; Micropropagation; BA; 2iP; TDZ; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究目的在建立原生金草蘭之微體繁殖技術。試驗以側芽為培植體,消毒方法為1%次氯酸納加入Tween 20消毒10分鐘。基本培養基為1/2 MS添加20 g/l蔗糖、2.5 g/l水晶洋菜、2 g/l活性炭或不加入活性炭,pH5.2。參試植物生長調節劑為BA、2iP、TDZ或組合Auxin (NAA) 與Cytokinins (BA、2iP或TDZ)。試驗結果顯示,初代微體繁殖培養4個月後,基本培養基添加5 ppm BA誘導不定芽發生頻率最高,添加TDZ增生之不定芽最多,其次為添加2iP,但添加TDZ增生之不定芽多為畸形芽,添加2 g/l活性炭誘導不定芽發生頻率會降低。經繼代培養於生長培養基 (基本培養基添加0.1 ppm NAA及3 ppm 2iP) 2次,每次2個月,此時添加TDZ增生之不定芽多數褐化。切取小苗置入發根培養基 (基本培養基添加30 g/l蔗糖、2 g/l活性炭、0.5 ppm NAA及1 ppm 2iP)培養1個月後,再移至基本培養基添加0.5 ppm IBA組合0.2 ppm BA、30 g/l蔗糖、2 g/l活性炭之發根培養基,培養1個月後,有不定根形成。經10個月的培養,以基本培養基添加3 ppm 2iP平均一個側芽培養可得到18.8個芽為最高。 |
英文摘要 | The objective of this study was to establish a micropropagation mothed for Dendrobium clavatum Lindl. Lateral bud was used as explant and sterilized with 1 % NaOCl + Tween 20 for 10-minute. Basal medium contained 1/2 MS medium, 20 g/l sucrose, 2.5 g/l gerlite, 2 g/l charcoal, and no charcoal at pH 5.2. Various media were prepared by basal medium supplemented with BA, 2iP, TDZ and combinations NAA. Through 4-month culture, the induction percentage of adventitious buds was found highest in basal medium + 5 ppm BA, but decreased in media with 2 g/l charcoal. Adventitious buds proliferated most in basal medium + TDZ and the next in basal medium + 2iP. However, most of the proliferated buds obtained from the medium with TDZ were found abnormal. Through two subculture with basal medium + 0.1 ppm NAA + 3 ppm 2iP, two months each, most of the proliferated buds in basal medium + TDZ became browning. Plantlets were transferred to the rooting media for 2-month, adventitious roots were formed. The recovered plants were hardened and planted in the greenhouse. In average, one lateral bud can be proliferated up to 18.8 adventitious buds in 10 month culture. |
本系統中英文摘要資訊取自各篇刊載內容。