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題名 | Quantitative Detection of ECP Level of STA-1 Treated HL-60 Cell by Using Real-time RT-PCR=應用Real-time RT-PCR檢測人類白血病細胞經STA-1培養後ECP之表現 |
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作者姓名(中文) | 張東迪; 許清祥; 黃介辰; | 書刊名 | 臺灣中醫醫學雜誌 |
卷期 | 5:1 民95.06 |
頁次 | 頁41-59 |
分類號 | 413 |
關鍵詞 | 即時反轉錄聚合酶鏈鎖反應; 中藥萃取物; 嗜伊紅性陽離子蛋白; 人類白血病細胞; RT-PCR; STA-1; ECP; HL-60; |
語文 | 英文(English) |
中文摘要 | 麥門冬湯與六味地黃丸之合方萃取物,STA-l能改善氣喘病患之臨床症狀及肺功能。本研究探討STA-l之抗發炎分子機轉。因此我們選擇ECP為標的,作為探討STA-l對此ECP表現之調控實驗,並因此藉以建立一細胞模型作為將來評估其它中藥調控ECP表現與發炎機轉之分子模型。本實驗將中藥,丁酸與人類白血病細胞HL-60 clone 15 cells一起培養2小時與24小時後,以相對定量real-time RT-PCR檢測ECP mRNA表現量,並以house keeping gene,β-actin,作內部定量控制(internal control)。實驗結果顯示,經由2小時與24小時的中藥、丁酸與HL-60 clone 15 cells培養後實驗組對ECP表現量之向上調控與對照組比較有統計上差異。本研究顯示以HL-60 clone 15 cells為材料,可作為監測ECP基因表現之很好模型。 |
英文摘要 | Though we have showed that the extracts of STA-l, a traditional Chinese medicine (TCM) formulae composed of Mai-Men-Dong-Tang (MMDT) and Lui-Wei-Di-Huang-Wan (LWDHW), could provide improvement in lung function and relieve asthma symptoms without adverse effects, molecular markers were needed to monitor the effect of STA-l on cell level. The gene of eosinophil cationic protein (ECP) was choose in this study to establish a model for investigating the effect of STA-l on ECP expression. The real-time RT-PCR was used to quantify the expression level of ECP mRNA levels in HL-60 clone 15 cells and a house keeping gene, β-actin, was used as the control for quantification. A significant expression of ECP by both STA-l and butyric acid treatment were showed during 24 hours culturing. The results suggest that expression level of ECP within HL-60 host cell may be a good candidate model to monitor the effect of Traditional Chinese Medicine, STA-l. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。