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題 名 | cDNA Cloning and Sequence Analysis of 5-Hydroxyconiferaldehyde O-Methyltransferase from Eucalyptus Camaldulensis=赤桉5-羥化針葉樹型乙醛O-甲基轉移酶(AldOMT)之互補核酸(cDNA)選殖及序列分析 |
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作 者 | 何政坤; 陳怡蒨; 陳振榮; | 書刊名 | 臺灣林業科學 |
卷 期 | 17:4 2002.12[民91.12] |
頁 次 | 頁429-438 |
分類號 | 436.182 |
關鍵詞 | 赤桉; 互補核酸cDNA選殖; 5-羥化針葉樹型乙醛O-甲基轉移酶; 咖啡酸O-甲基轉移酶; 木質素專一性基因; Eucalyptus camaldulensis; cDNA cloning; 5-hydroxyconiferaldehyde O-methyltransferase; Caffeate O-methyltransferase; |
語 文 | 英文(English) |
中文摘要 | 從赤胺樹幹刮取發育中的次生木質部組織,並萃取總量RNA,再利用反轉錄之合 成醃連鎖反應(RT-PCR),擴大複製出一條l10l bp編碼製造5-羥化針葉樹型乙醛O-甲基轉移 ?(5-hydroxyconiferaldehyde O-methyltransferase; AldOMT)之互補核酸片段(REOMT-ll) ,隨後將其選殖到PCRII TOPO載體。經過核酸定序,REOMT-ll含有1098 bp的開放閱讚框( open reading frame),可轉譯成366個胺基酸的一條多胜?,其分子量的估測值為39,900 。接著再利用合成?連鎖反應,擴大複製5'端上游與3'端下游的核酸片段,而獲得全長度赤 桉AldOMT cDNA之選殖, 並定名為EucOMT1。經胺基酸序列比對,選殖的EucOMTI明顯歸屬 於caffeate O-methyltransferase (COMT)類型,而與caffcoy]-CoAO-methyltransferase( CCoAOMT)之類型相距甚遠。進一步的分析,發現EucOMW具有4個可能連接S-adenosyl-L- methionine(SAM)的區段。 |
英文摘要 | Total RNA of developing secondary xylem scraped from the woody stem of Eucalyptus camaldulensis was extracted and used as initial material for cloning. A 1101-bp cDNA fragment (REOMT-11) encoding 5-hydroxyconiferaldehyde 0-methyltransferase (AldOMT) was amplified using reverse-transcription polymerase chain reaction (RT-PCR), and cloned into a PCRll TOPO vector. Nucleotide sequencing revealed that the REOMT-11 clone contained an open reading frame of 1098-bp cDNA, which encoded a polypeptide of 366 amino acid residues with a predicted molecular weight of 39,900. A full-length AldOMT cDNA clone (EucOMT1) was obtained by supplementing 2 other fragments, which were amplified from upstream and downstream regions of the REOMT-11 clone. In comparisons of deduced amino acid sequences, EucOMT1 was classified into the type of caffeate 0-methytransferase (COMT) which used to be defined as catalyzing the methylation of caffeic acid to ferulic acid, and away from the caffeoyl-CoA 0-methyltransferase (CCoAOMT) class. In further analyses, 4 possible S-adenosyl-L-methionine (SAM) binding domains were found in the deduced amino acid sequence of the EucOMT1 clone. |
本系統中英文摘要資訊取自各篇刊載內容。