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題 名 | 利用核醣體核酸內轉錄間隔區鑑別二種露菌病菌=Identification of Two Downy Mildew Fungi in Taiwan Based on the Internal Transcribed Spacer of the Ribosomal Region |
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作 者 | 陳立祥; 鄭奇煒; 陳瑞祥; 蔡竹固; | 書刊名 | 植物保護學會會刊 |
卷 期 | 48:1 民95.03 |
頁 次 | 頁53-64 |
分類號 | 433.4 |
關鍵詞 | 露菌病; 內轉錄間隔區; 聚合酶連鎖反應; Peronospora parasitica; Pseudoperonospora cubensis; Downy mildew; Internal transcribed spacer; ITS; Polymerase chain reaction; |
語 文 | 中文(Chinese) |
中文摘要 | eronospora parasitica 和 Pseudoperonospora cubensis 以引子對 ITS1/ITS4 ,經由聚合?連鎖反應( PCR )皆可增幅出約 890 bp 片段。 將此二菌 DNA 片段 解序後 ,分別 設計專一性引子對 PP1/PP2 及 PsC1/PsC2 。以 PP1/PP2 經由 PCR 用於偵測 P . parasitica 時,可在感染露菌病的油菜葉片得到一預期 514 bp 產物,而無法自 P. manshurica 、 P. tabacina 、 Ps . cubensis 及 Plasmopara viticola 增幅出任何產物。以引子對 PsC1/PsC2 經由 PCR 用於偵測 P . cubensis 時,可在感染露菌病的胡瓜葉片得到一預期 505 bp 產物,無法自 P. parasitica 、 P. tabacina 、 Pl . viticola 增幅出預期產物;但仍可自 P. manshurica 增幅出近乎同等長度之 rDNA 產物。進一步將 PCR 產物以 Dde I 進行?切之後,可在 P. manshurica 得到 449 和 57 bp 條帶,而 Ps . cubensis 則不具有該限制?的識別序列位置,故亦可加以進一步鑑別。 |
英文摘要 | The roles of both ammonium stress and cytokinin effect, played in the microshoot induction of rice by phosphinothricin (PPT)(1) have been studied. More microshoots were produced by the treatment combination of 100 mM ammonium sulfate applied at stage I and 50 mM at stage II of in vitro culture of rice buds, and this effect was significantly enhanced by benzyladenine (BA). Anatomical study indicated that the number of lateral buds was slightly increased under the high ammonium stress, and it was largely increased when coupled with BA treatment. This suggests that the primordia of lateral buds were induced by ammonium through the interruption of apical dominance. But the subsequent growth was possibly hampered by the high ammonium concentration in tissues, unless this effect was overcome by BA. The large increase of peroxidase activity, an indicator of cell division, in apical dome of microshoot 10 days after BA treatment, might support the subsequent growth of lateral buds. In addition, the successive increase of three key amino acids in glutamine synthetase/glutamate synthase (GS/GOGAT; EC 1.4.1.3) pathway, i.e., glutamine, glutamate and glycine, by BA, might be advantageous to the recovery of microshoot growth hampered by ammonium stress. |
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