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頁籤選單縮合
題名 | Polymerase Chain Reaction Identification of a Hymenopteran Insect in the Cornea: A Case Report=以聚合酶連鎖反應(PCR)確認角膜上之膜翅目昆蟲一病例報告 |
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作者姓名(中文) | 林憲忠; 葉文斌; 張丞賢; 傅耀賢; 程建中; 吳弘鈞; 王惠珠; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
卷期 | 22:3 民95.03 |
頁次 | 頁143-148 |
分類號 | 416.711 |
關鍵詞 | 角膜昆蟲異物; 聚合酶連鎖反應; 膜翅目; Corneal insect foreign body; Polymerase chain reaction; Hymenoptera; |
語文 | 英文(English) |
中文摘要 | 昆蟲異物引起的角膜反應與該異物的成份有關。然而,之前報告過的角膜昆蟲異物很少以科學的方法佐證。 我們提出一位 49 歲男性,其左眼遭一未知之昆蟲撞擊。經緊急角膜切開術取出異物後,將檢體置於光學顯微鏡下觀察並作分子分析。將聚合連鎖反應 (PCR) 之產物作排序並比對,屬於膜翅目 (Hymenoptera) 昆蟲姬蜂總科的近緣物種。 |
英文摘要 | The type of corneal injuries associated with insect encounters is related to the composition of the foreign body. However, previous reports on corneal foreign bodies as insects were rarely based on scientific evidence. Here, we report on a 49-year-old male who was stung in his left eye by an unknown insect. Emergency keratotomy was performed to remove the embedded corneal foreign body. The removed foreign body was observed under light microscopy, and a fragment of insect was suspected. The sample was sent for molecular analysis. The polymerase chain reaction product was sequenced, subjected to a BLAST search, and identified as an ichneumonoid member of the insect order Hymenoptera. |
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