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題名 | 富含粒線體細胞在斑馬魚發育過程中的表現=Distribution of Mitochondria-Rich Cells in Developing Zebrafish |
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作者姓名(中文) | 林豊益; 楊以屏; 洪君琳; | 書刊名 | 樹人學報 |
卷期 | 3 民94.07 |
頁次 | 頁183-194 |
分類號 | 388.5 |
關鍵詞 | 斑馬魚; 胚胎; 富含粒線體細胞; 離子運送; Zebrafish; Embryo; Mitochondria-rich cell; Ion transport; |
語文 | 中文(Chinese) |
中文摘要 | 離子調節是動物維持生理恆定的最重要項目之一。人體的腎臟是用以調節血液中各種離子濃度恆定的主要器官。基礎研究上,培養之哺乳類腎臟上皮細胞、兩生類及魚類的表皮已廣被作為研究上皮離子運送的材料。斑馬魚胚胎是發育生物學當中所廣為使用的模式動物,許多研究報告更指出斑馬魚將會繼果蠅後成為遺傳學上最重要的模式物種。本研究以斑馬魚為研究對象,探討在發育中之斑馬魚胚胎是否有調節離子的MR細胞,並探討是否斑馬魚具有作為研究此類細胞的有利特性。利用掃描式電子顯微鏡與細胞免疫染色技術進行斑馬魚體表MR細胞的分析,結果發現在斑馬魚胚胎發育過程中,上皮MR細胞分布於胚胎的表皮中。因此本研究推論利用斑馬魚這種模式動物,將可作為研究上皮離子運送細胞的有利材料。 |
英文摘要 | Ion-regulation is one of the important physiological events in body homeostasis of animals. In human, kidneys play the major role of maintaining ionic constancy of body fluid. Cultured epithelium of mammalian kidneys, amphibian skin and fish branchial epithelium are the powerful models for the researchs on ion- and osmo-regulation physiology. Previously, embryos of zebrafish have been well established to be an important model animal in developmental biology and genetics. The purpose of this study was to establish a new and ideal model for studying epithelial ion-transport. In this study, zebrafish embryos were examined with immunocytochemistry and scanning electron microscopy, and the epithelial ionocytes, mitochondria-rich (MR) cells were found in the skin epithelium of developing embryos. This work suggests that zebrafish embryo could be a potential model for studying ion-transporting epithelium. |
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