頁籤選單縮合
題名 | Heparin Modulates Human Osteosarcoma (MG-63) Activities on Collagen-coated Substrata=肝素對類骨母細胞(MG-63)在膠原蛋白基材上的影響 |
---|---|
作者 | 蔡曉雯; 鄭育慧; 蔡偉博; Tsai, Shiao-wen; Cheng, Yu-hui; Tsai, Wei-bor; |
期刊 | Journal of the Chinese Institute of Chemical Engineers |
出版日期 | 20051100 |
卷期 | 36:6 民94.11 |
頁次 | 頁661-668 |
分類號 | 346.77 |
語文 | eng |
關鍵詞 | 肝素; 骨母細胞; 生醫材料; 膠原蛋白; Collagen; Heparin; Osteoblast; Cell adhesion; |
中文摘要 | 膠原蛋白是細胞外基質的主要蛋白質,也因為其優越的生物相容性,成為受歡迎的生醫材料。膠原蛋白被廣泛於外科手術縫線、繃帶、藥物傳輸用水膠、傷口敷材,也應用於新近興起的組織工程領域。如果對於細胞和膠原蛋白基材,以及其他的細胞外基質成分的交互作用有深一層的瞭解,應該對於設計組織工程用鷹架有進一步的幫助。在本研究中,我們探討肝素在膠原蛋白基材上,對於骨母細胞的影響。膠原蛋白(0.1 mg/mL)和不同濃度的肝素(0.02 mg/mL、0.1 mg/mL和mg/mL)混合後,塗佈在細胞培養盤中。我們發現低濃度肝素(0.02mg/mL)能夠提高骨母細胞的附著和伸展,但是對於細胞生長和鹼性磷性磷酸 的活性沒有影響。相反地,高濃度的肝度(0.1和0.5 mg/mL)降低骨母細胞的附著和伸展,同時也減少細胞的繁殖。本研究的結果顯示少量的肝素可以增加細胞的貼附與伸展,但是高濃度的肝素並不適合細胞培養。 |
英文摘要 | Collagen, the major extracellular matrix component, is a popular biomaterial due to its excellent biocompatibility. Collagen is widely used in sutures, corneal bandages, drug delivery gels, and as wound dressing, as well as in a newly emerging interdisciplinary biomedical field, tissue engineering. A better understanding of the interaction between cells and collagen substrata combining other ECM components should allow for a more rational scaffold design for tissue engineering. In this study, we explored the effects of heparin on the behavior of osteoblast-like cell lines (MG-63) on collagen-based substrates. Collagen mixture (0.1 mg/mL) with heparin at different concentrations (0.02 mg/mL, 0.1 mg/mL, and 0.5 mg/mL) was coated onto tissue culture polystyrene plates. We found that low concentration of heparin (0.02 mg/mL) enhanced cell adhesion and spreading on collagen-coated plates, but did not influence cell growth and alkaline phosphates activity. On the other hand, high doses of heparin (0.1 and 0.5 mg/mL) decreased cell adhesion and spreading, as well as cell proliferation. Our results suggest that a small amount of heparin might support osteoblast attachment and spreading although high heparin concentration is not suitable for cell culture. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。