查詢結果分析
來源資料
相關文獻
- Repeated DNA Vaccination Induced Neonatal Tolerance Against Escherichia. Coli. Beta-Galactosidase in Immune-Competent Mice
- DNA疫苗--傳染病的未來剋星
- DNA疫苗之研究及應用
- DNA疫苗
- Analysis of Humoral Immunity of Hepatitis D Virus DNA Vaccine Generated in Mice by Using Different Dosage, Gene Gun Immunization, and in Vivo Electroporation
- 登革重組次單位疫苗的發展與挑戰
- 以生物晶片技術應用於研發DNA疫苗
- Anti-CTLA4 scFv, a Single--Chain Antibody, Can Prolong Expression of Escherichia. coli. beta-Galactosidase in Immune--Competent Mice
- 發展中的流感疫苗
- 「DNA疫苗」的發展
頁籤選單縮合
題名 | Repeated DNA Vaccination Induced Neonatal Tolerance Against Escherichia. Coli. Beta-Galactosidase in Immune-Competent Mice=重覆DNA疫苗注射能誘發免疫力正常的小黑鼠對大腸桿菌半乳糖酶產生免疫耐受性 |
---|---|
作者 | 劉安邦; 戴國峰; 林欣榮; | 書刊名 | 慈濟醫學 |
卷期 | 17:2 民94.04 |
頁次 | 頁75-81+130 |
分類號 | 414.82 |
關鍵詞 | 轉植基因; 新生兒免疫耐受性; DNA疫苗; Transgene; Neonatal tolerance; DNA vaccination; |
語文 | 英文(English) |
中文摘要 | 本研究的目的是要探討以重覆DNA疫苗注射能否誘發免疫力正常的小黑鼠對大腸桿菌半乳糖酶產生新生兒免疫耐受性。材料與方法:我們取兩組初生的C57BL/6小黑鼠,每組六隻,一省一在其後頸皮下注射2微克(μg)溶於無菌磷酸鹽緩衝溶液不含細菌內毒素的質體-pCBLacZ,另一組經由肛門注射等量pCBLacZ。每一隻新生小在出生後第二天接受注射,以後每隔七天注射一次,一共注射六次,在最後一次注射後七天,所有小鼠都在左前脛骨肌注射50微克的pCBLacZ,這個質體將表達大腸桿菌半乳酶以誘發局部的免疫反應。對照組則有六隻未曾接受過任何處置的六週大C57BL/6成鼠在左前脛骨肌接受50μg pCBLacZ的肌肉注射。結果:我們在注射後第六天檢視小鼠的肌肉切片,發呈現半乳酶活性的肌細胞數目在對照組平均為16.2,經皮下注射DNA疫苗者為0,由肛門注射為168.2,以Likelihood ratio test分析得p值為0.0001,在肛門注射組中有兩隻小鼠分別有409和513個肌細胞表達半乳酶;以ELISA分析三組血中抗半乳酶IgG含量,則發現接受肛門注射的小鼠其血中IgG要比其它兩省一來得低。結論:在新生小鼠給予重覆DNA疫苗注射能引發免力正常的小鼠對異蛋白產生免疫耐受性,同時疫苗的投予方式會決定耐受性的產生與否。 |
英文摘要 | Objectives: To induce neonatal immune tolerance in immune competent animals by repeated DNA vaccination. Materials and Methods: We use two groups of neonatal immune competent mice, 576, six in each. Mice in one study group received subcutaneous injection of 2μg of endotoxin-free plasmid, pCBLacZ, at the posterior neck area. Concentration of the plasmid was 0.5 mg/mL. This plasmid encoded a potent immunogen, Escherichia coli beta-galactosidase. Mice in the other study group received intraectal injection of same amount of pCBLacZ. All the animals received injection at the second day of birth and 5 consecutive injections of the same does of pCBLacZ at an interval of 7 days. Seven days after the last shoot, the mice received an intramuscular injection of 50μg of endotoxin-free pCBLacZ at a concentration of 2mg/mL at the left anterior tibialis. We used 6 native adult 42days. Results: We examined the expression of beta-galactosidase in muscle fibers 6 days after the last intramuscular injection. The average numbers of fibers expressing beta-galactosiase was 168.2 in the mice receiving interracial immunization as cored with 0 in the mice receiving subcutaneous injection and 16.2 in their little mates. There were tow mice got 409 and 518 blue fibers respectively. Serum IgG against the beta-galactosidase was lower in the animals receiving interracial injection as compared with the mice in the other two gropes by nonprametric Mann-Whitney test. Conclusions: Repeated DNA vaccination in the neonatal stage can induce immune tolerance against a potent immunogen in the immune competent animals. The ways of administration determine the success of tolerance induction. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。