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題名 | In Vitro Induction of Phosphinothricin Tolerance in Rice (Oryza Sativa)=利用Phosphinothricin(PPT)於試管內誘導水稻產生PPT耐性植株 |
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作者姓名(中文) | 劉育妏; 周煒裕; 王慶裕; | 書刊名 | 植物保護學會會刊 |
卷期 | 47:1 民94.03 |
頁次 | 頁47-58 |
分類號 | 434.111 |
關鍵詞 | 耐性; 水稻; 微芽; Phosphinothricin; Tolerant; Rice; Microshoot; PPT; |
語文 | 英文(English) |
中文摘要 | 利用三階段培養方式使水稻幼芽產生耐 phosphinothricin (PPT; glufosinate)的微芽(microshoots)。在第一階段的組織培養中,水稻越光品種其幼芽在處理0.76 μM以上的PPT時生長受到抑制,且小芽比大芽敏感。經28天的培養後, 在5.00 μM處理下較其他處理有最佳的微芽誘導效果,但高濃度PPT會導致植株死亡。在第二階段,所有經第一階段處理而來的芽體,皆去頂維持芽高15 mm並繼代於含5.00 μM PPT的培養基中30天。結果顯示,第一階段以對照組的植株,在經過第二階段5.00 μM PPT處理後反而具有較佳的微芽誘導能力。第二階段所產生的所有芽體再進一步繼代於分別含0.0、5.0及10.0 μM PPT的培養基中,經30天後雖然有更多的芽體因高濃度的PPT處理而死亡,但第三階段在10.00 μM PPT處理下的植株卻誘導出更多的芽體,隨後繼代於不含PPT的培養基15天後仍有芽體死亡。雖然不同PPT處理組合下存活芽體長成的植株,在以0.75及1.51 mM PPT進行噴藥篩選時PPT耐性表現並無明顯差異,但依134株植株傷害指數的頻度分布中二極端值可以選出對PPT感性與耐性的植株11株,進一步以葉片片段檢定方式可以從中選出兩個較感性與兩個較耐性植株。 |
英文摘要 | In vitro culture of rice buds has been developed to induce tolerance to phos- phinothricin (PPT), i.e., the glufosinate herbicide. Treatment at stage I of tissue culture with PPT concentrations higher than 0.76 μM inhibited the bud growth of rice cv. Koshihikari, with smaller buds being more sensitive. Through 28-day incubation, microshoot induction was most significantly stimulated by 5.0 μM PPT, as compared with other concentrations; with higher levels resulting in plant death. At stage II of tissue culture, all rice plantlets were decapitated to 15 mm tall and incubated for 30 days in hormone-free medium containing 5.0 μM PPT. Rice buds which were cultured PPT-free at the first stage, and followed by 5.0 μM PPT treatment, generated a higher induction rate of microshoot than those treated at both stages. At stage II, plantlets that survived 30 days after treatment of 10.0 μM PPT produced more microshoots than those treated with no or 5.0 mM PPT. While no significant difference in tolerance with a foliar-application of PPT at either 0.75 or 1.51 mM was observed in microshoot-derived plants which had survived from 9 different PPT treatment combinations, 11 plants could be referred as the PPT-tolerant and -susceptible plants, based on the both extremes of frequency distribution of injury index of all plants. Two tolerant plants and two susceptible plants have been selected by means of leaf segment assay. |
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