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頁籤選單縮合
題 名 | Study on the Detection Method of Six Varieties of Genetically Modified Maize and Processed Foods=檢測六種品系基因改造玉米及其加工製品方法之探討 |
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作 者 | 闕麗卿; 陳彥伶; 施養志; | 書刊名 | 藥物食品分析 |
卷 期 | 10:1 2002.03[民91.03] |
頁 次 | 頁25-33 |
分類號 | 412.37 |
關鍵詞 | 聚合酵素鏈反應; 基因改造玉米; PCR; GM-maize; |
語 文 | 英文(English) |
中文摘要 | 本研究以 PCR 方法鑑別檢測六種品系基因改造玉米 (genetically modified maize,簡稱 GM玉米)。首先探討加熱處理對於 PCR 檢測之影響,玉米分別經由100°C及121°C不同時問熱處理,續抽取 DNA ,以玉米zein基因引子進行 PCR 檢測,結果發現100°C處理120 min與121°C 處理30 min仍可檢到出zein基因。次為建立六種品系 GM-玉米之PCR鑑別檢測方法,針對Event 176 ( Novartis 公司)、Bt11 ( Novartis 公司)、MON810 ( Monsanto 公司)、 T25 ( AgrEvo 公司)、 CBH-351 ( AgrEvo 公司)及 GA21 ( klousalito 公司) GM-玉米之殖入基因分別選定具產品特異性引子,進行 PCR 方法檢到,其最低檢測濃度皆小於0.1% (w/w)。為瞭解食品玉米原料及加工玉米製品含 GM玉米之狀況,採用前述建立方法到試市售食品玉米原料、冷凍玉米粒、玉米粒罐頭、墨西哥玉米餅及洋芋片等檢體。結果發現玉米碎同件檢體及墨西哥玉米餅同件檢體可分別檢出六種及五種測試品系 GM-玉米;冷凍及罐頭玉米粒可檢出 MON810 及 T25 二種GM-玉米;洋芋片檢體則含有MON810 GM-玉米。綜合上述, PCR 方法適用於玉米原料及其製品之檢測,並得知台灣目前市售玉米原料及其製品含有混合之不同品系 GM-玉米,並以 MON810 GM玉米品系為大宗。 |
英文摘要 | To detect and identify six varieties of genetically modified (GM) maize, polymerase chain reaction (PCR) assays were performed in the present study. The effect of heat treatment on the detection of the genes by the PCR methods was investigated. Maize samples were subjected to 100°C and 121°C heat treatment for different time intervals, and PCR tests were then conducted by using primers specific for the maize zein gene. Results revealed that the zein gene still could be detected by the PCR after heating at 100°C for 120 min and heating at 121°C for 30 min. In addition, the establishment of PCR assays on the identification of the six varieties of GM-maize was also evaluated. Primers specific for the inserted genes in the Event 176 (Novartis), Bt11 (Novartis), MON810 (Monsanto), T25 (AgrEvo), CBH-351 (AgrEvo), and GA21 (Monsanto) GM-maize were used to conduct the PCR assays. The detection limits for these GM-maize crops is less than 0.1% (w/w). For further understanding on whether commercially available maize materials and processed foods are mixed with GMmaize, corn middling pellets, canned whole kernel maize, frozen whole kernel maize, corn tortilla and potato chips samples were collected from the markets, and detected by the PCR methods developed in this study. Results showed that five to six varieties of GM-maize were detected in the corn middling pellets and corn tortilla, whereas GM-maize of the MON810 variety as well as the T25 variety were detected in canned and frozen whole kernel maize samples. Potato chips only contained GM-maize of the MON810 variety. The results of this study demonstrate that the PCR analysis is suitable for detecting maize materials and products, and that commercially available maize materials and products in Taiwan are usually mixed with different varieties of GM-maize. Meanwhile, the major product of GM-maize is the MON810 variety. |
本系統中英文摘要資訊取自各篇刊載內容。