頁籤選單縮合
題 名 | 氨氮無氧氧化菌群馴養特性及微生物菌相分析=DGGE Analysis and Cultivation for Anaerobic Ammonia Oxidizing Bacterial Community |
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作 者 | 謝淵琳; 蔡慧穎; 張裕釧; 林畢修平; | 書刊名 | 中華生質能源學會會誌 |
卷 期 | 23:3/4 民93.12 |
頁 次 | 頁61-68 |
分類號 | 445.463 |
關鍵詞 | 氨氮; 氨氮無氧氧化; Ammonia; Anaerobic ammonia oxidation; SBR; DGGE; Candidatus Brocadia anammoxidans; |
語 文 | 中文(Chinese) |
中文摘要 | 氨氮無氧氧化反應是藉由特殊微生物直接將NH₄+-N與NO₂¯-N反應成為N₂、NO、及N₂O等氣態氮,不需加碳源,也不需要曝氧氣,能在單一反應槽中去除廢水含氮物質,可減少有機碳、電力能源及土地的支出。本研究目的是以循序批式反應槽(sequencing batch reactor, SBR)進行氨氮無氧化菌群(anaerobic ammonium oxidizing bacteria, ANAMMOX bacteria)的馴養。連續監測馴養槽效能以建立其穩定性,並進行定期採樣,應用變性梯度膠體電泳(denaturant gradient gel electrophoresis, DGGE)方法,解析槽內細菌族群的變化。因氨氮無氧化菌群需於無碳系統馴養,菌群生長緩慢;馴養槽自第80天起NH₄+-N及NO₂¯-N有明顯去除現象,至第114天馴養槽出流水之NH₄+-N、NO₂¯-N濃度分別為 0.4 mg-N/l 及 0.25 mg-N/l,並含有 6.30 mg-N/l 之 NO₃¯-N,總氮去除率達87.8%。此馴養槽之氨氮無氧氧化菌群污泥樣本,在變性梯度膠體電泳分析下呈現菌相單純化的趨勢。馴氧槽生物污泥樣本經PCR反應、進行 16S rRNA 基因定序比對結果,顯示污泥中菌株與 Candidatus Brocadia anammoxidans 有97%相似度。根據馴氧槽第80天至114天的水質分析數據及菌群的分析結果,顯示此馴養槽內已馴養出本土性之氨氮無氧氧化微生物。 |
英文摘要 | Anaerobic ammonia oxidation reaction takes place with the application of an unique microorganism which can generate N2, NO and N2O from NH4+-N and NO2--N wihout organic carbon and aeration. It could save the cost of organic carbon, electricity and land. The aim of our study is using a lab-scale sequencing batch reactor (SBR) to cultivate anaerobic ammonium oxidation (ANAMMOX) bacteria for the treatment of nitrogen contamination in wastewater. The efficiency of the reactor was continuously monitored. Sludge samples from SBR were investigated by both polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) methods. It was reported that ANAMMOX cell can grow in non-oxygen and non-organic substrate, therefore, the SBR reached the state of anaerobic condition by filling 95% argon / 5% fo CO2 gas and on the other hand, artificial substrate was used as carbonless media. The SBR presented NH4+-N and NO2--N elimination ability after 80 days of cultivation and the out flow concentration of NH4+-N, NO2 --N and NO3--N reached as low as 0.4 mg N/l, 0.25 mg N/l and 6.30 mg N/l, respectively. In addition, the total nitrogen removal rate was 87.8% at the 114th day. The bacteria community trended toward uncomplicated on DGGE gel. The result of 16SrRNA gene analysis shows that a species in the sludge has 97% gene similarity with Candidatus Brocadia anammoxidans. According to the outcome of water and microbial analysis, the SBR has successfully cultivated a local strain of ANAMMOX bacteria and their application will be developed in the near future. |
本系統中英文摘要資訊取自各篇刊載內容。