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題名 | 半枝蓮中抗腫瘤活性成份之萃取研究=Extraction of Anti-Tumor Activities from Scutellaria Barbata Don |
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作者 | 魏明治; 韓建華; 楊竹茂; 陳莉螢; | 書刊名 | 嘉南學報. 科技類 |
卷期 | 30 民93.12 |
頁次 | 頁65-74 |
分類號 | 414.332 |
關鍵詞 | 半枝蓮; 超臨界二氧化碳萃取; 溶劑萃取; Scutellaria barbata don; Supercritical carbon dioxide extraction; Solvent extraction; Wogonin; Baicalein; Baicalin; |
語文 | 中文(Chinese) |
中文摘要 | 本研究以溶劑與超臨界二氧化碳,分別萃取半枝蓮中的naringenin、luteolin、apigenin、wogonin、baicalein、baicalin,以及protocatechuic acid等保肝與抗腫瘤成份。探討各種萃取條件,如不同溶劑(水、乙醇、丙酮及正己烷等)、萃取的溫度與時間,以及超臨界二氧化碳萃取的溫度與壓力等變因對於各成份萃取量之影響。各種萃取條件之萃取液經由高效率液相層析儀(HPLC)定量分析,以作為最佳萃取條件之指標。在溶劑萃取方面,顯示以40-80%的乙醇與50%丙酮水溶液作為萃取劑,在50℃下萃取1小時,naringenin等六種黃酮類成份具有較佳的萃取產率。對於protocatechuic acid而言,其萃取產率於純水中較高。於壓力117-154 bar與溫度40-50℃下,以超臨界二氧化碳萃取半枝蓮時,其萃取效果均不佳。 |
英文摘要 | This study extracted the hepatoprotective and antitumor activities, such as naringenin, luteolin, protocatechuic acid, wogonin, baicalein, baicalin and apigenin from Scutellaria Barbata Don using solvent and supercritical carbon dioxide extraction. With pure water, different percentage of alcohol aquenous solution, acetone and n-hexane as extractants, the effects of extraction factors, temperature and extraction time on extraction yield were studied. On the other hand, the effects of temperature and pressure on extraction yield were also discussed using supercritical fluid extraction. Analyses of the extracts were performed by high-performance liquid chromatography to determine the contents of components. For all the analytes of the experimental conditions providing the highest extraction yield inside the experimental domain considered were found. Solvent extraction results showed that the optimum conditions for six flavonids constitutions, such as naringenin etc. were achieved by using 40-80% ethanol and 50% acetone at 50℃ for extraction 1 h. The content of protocatechuic acid in ethanol and acetone extracts was low, but water extracts showed higher content. On the other hand, using carbon dioxide alone in supercritical fluid extraction at pressures from 117 to 154 bar and temperatures from 40 to 50℃, the extraction yield of Schtellaria Barbata Don was not satisfactory. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。