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頁籤選單縮合
題名 | 利用大豆廢水進行黑殭菌菌絲體最適化培養之探討=Optimal Production of Mycelia of Metarhizium Anisopliae var. Anisopliae by Using the Waste of Soy Water |
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作者姓名(中文) | 謝建元; 薛一祥; 羅朝村; 高穗生; |
作者姓名(外文) | Hsieh, Chienyan; Hsieh, Yi-hsiang; Lo, Chaur-tsuen; Kao, Suey-sheng; |
書刊名 | 南臺科技大學學報 |
卷期 | 29 2004.12[民93.12] |
頁次 | 頁113-126 |
分類號 | 433.3 |
語文 | chi |
關鍵詞 | 大豆廢水; 黑殭菌; 回應曲面法; Soy waste water; Metarhizium anisopliae; Response surface method; RSM; |
中文摘要 | 蟲生真菌黑殭菌有很廣的昆蟲宿主範圍,是一極具商品化潛力之殺蟲微生物。本研究利用大豆廢液對黑殭菌進行培養,在搖瓶中以回應曲面法進行最適化基質探討,並應用於5公升發酵槽進行其他因廣在生產菌絲體之探討。在搖瓶試驗中,以豆水、糖蜜、鹽類與pH調整,進行探討,經由回應曲面分析法之二階數學模式尋得最適培養基為98.4%豆水、5.68%糖蜜、pH6.54可得最大生產量為20.85g/L。在5公升醱酵槽培養中,不同轉速下菌絲體有不同的菌體型態產生,在越高轉速攪拌下,其形成的菌絲球越小且密度越高。培養條件為28℃、350rpm通氣量分別為1 vvm及2 vvm通氣量的菌體濃度分別可獲得菌體濃度28.5g/L和30.5g/L。本研究結果,使用人豆廢水在低成本下生產黑殭菌,可在短時間得到很高菌體產量,使黑殭菌商品化中程為極具競爭力之殺蟲微生物。 |
英文摘要 | The entomothogenic fungus Metarhizium anisopliae parasitizes a wide range of insect hosts and had great potential been utilized as the biology control agent. The purpose of this study is to use the waste water of tofu manufacturing for the culturing M. anisopliae in submerged fermentation. In shake-flask, response surface method (RSM) was used to search the media for optimal growing conditions of 3t. anisopliae and then applied this media in a 5-L jar fermentor for further growth study. In shake-flask tour factors included soy water, molasses, salts, and pH were used in this analysis. The optimal media from the second order polynomial equation as the results of RSM is 98.4% soy water, 5.68% molasses, pH 6.54, and one time of basal salts. The maximum production of cell concentration is 20.85 g/L. In 5-L jar fermentor different mycelia morphology was found with various agitation speeds. With the high speed of agitation the mycelia was resulted in higher concentration of cell and pellet but smaller size of pellet. Applied this media with the conditions of 28 ℃, 350 rpm, and the aeration of 1 vvm and 2 vvm resulted in cell concentration of 28.5 g/L and 30.5 g/L, respectively. Results of this study were able to use soy waste water with low cost raw material to mass production of M. anisopliae. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。