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題名 | Effects of Hyperlipidemia on Aortic Endothelial Cell Turnover and Transendothelial Macromolecular Transport in Cholesterol-Fed Rats=在餵食膽固醇老鼠之高脂血症對於動脈內皮細胞週轉和穿內皮細胞層大分子物質輸送的影響 |
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作者 | 林幸榮; 丁燿宗; Lin, Shing-jong; Ding, Yaw-zon; |
期刊 | 中華醫學雜誌 |
出版日期 | 19961000 |
卷期 | 58:4 1996.10[民85.10] |
頁次 | 頁235-240 |
分類號 | 415.38 |
語文 | eng |
關鍵詞 | 動脈硬化症; 細胞死亡; 細胞分裂; 內皮細胞; 伊文斯藍-白蛋白; 高脂血症; 通透性; Atherosclerosis; Cell death; Cell mitosis; Endothelium; Evans blue-albumin; Hyperlipidemia; Permeability; |
中文摘要 | 背景 高脂血症、高血壓、吸煙和糖尿病是動脈硬化症和心臟血管疾 病的主要危險因子。重複的內皮細胞損傷和增強血漿脂蛋白流入局部血管內膜 是動脈硬化生成的主要病生理機轉。我們先前的研究發現在自發性高血壓老鼠, 長期飲用含尼古丁水的老鼠和Streptozotocin誘發糖尿病老鼠的主動脈內皮細胞 週轉和伴隨的內皮細胞通透性有明顯增加的現象。在本研究中,我們探討在餵 食膽固醇老鼠之高脂血症對於動脈內皮細胞週轉和穿內皮細胞層大分子物質輸 送的影響。 方法 16隻雄性Sprague-Dawley老鼠給予餵食含5%膽固醇的飼料達6週。另 外14隻同年齡的老鼠給予餵食一般的飼料相同期間做為對照組。在胸主動脈的 正面標本上,利用蘇木素染色來找出分裂中的內皮細胞,利用間接免疫過氧化 脢法找出含IgG死亡的內皮細胞,同時利用螢光顯微鏡檢術來定量內皮細胞層 對於伊文期藍-白蛋白複合物的洩漏程度。 結果 餵食膽固醇的老鼠有較高的血漿膽固醇和三酸甘油脂濃度。和對照組老 鼠比較,高脂血症老鼠的主動脈有較高的內皮細胞死亡頻率(1.08 +/- 0.28%比0.75 +/- 0.16%),較高的內皮細胞分裂頻率(0.015 +/- 0.005%比0.013 +/- 0.003%)和較大 的伊文期藍-白蛋白洩漏位置之數目密度(6.19 +/- 0.64/平方毫米比5.23 +/- 0.76/ 平方毫米)。 結論 與高血壓,飲用尼古丁水和糖尿病的情況類似,在餵食膽固醇老鼠的主 動脈中,內皮細胞週轉頻率和內皮細胞層對大分子物質通透性的增加可能是高 脂血症加速動脈硬化生成的主要機轉。 |
英文摘要 | Background. Hyperlipidemia, hypertension, cigarette smoking and diabetes are some major risk factors for atherosclerosis and cardiovascular disease. Repeated endothelial injury and enhanced focal intimal influx of plasma lipoproteins are the pivotal mechanisms involved in atherogenesis. We previously demonstrated that the endothelial cell turnover and associated endothelial permeability were significantly increased in the aorta of spontaneously hypertensive rats, chronic oral nicotine-treated rats, and streptozotocin-diabetic rats. In the present investigation, we examined the effects of hyperlipidemia on arterial endothelial cell turnover and transendothelial macromolecular transport in cholesterol-fed rats. Methods. Sixteen male Sprague-Dawley rats were fed a diet containing 5% cholesterol for 6 weeks. A group of 14 age-matched rats fed a regular diet and maintained over the same time period, were served as the controls. In en face preparations of the thoracic aorta, mitotic endothelial cells were identified by hematoxylin staining, IgG-containing dead endothelial cells were identified by an indirect immunoperoxidase method, and endothelial leakage to Evans blue- albumin complexes was quantified by fluorescence microscopy. Results. The results showed that plasma cholesterol and triglyceride levels were higher in cholesterol-fed rats. The hyperlipidemic rats, compared to control rats, had higher values for the frequency of endothelial cell death (1.08 +/- 0.28% vs 0.75 +/- 0.16%), the frequency of endothelial cell mitosis (0.015 +/- 0.005% vs 0.013 +/- 0.003%) and the number density of Evans blue-albumin leaky foci (6.19 +/- 0.64/mm2 vs 5.23 +/- 0.76/mm2) in the aorta. Conclusions. Similar to the situations in hypertension, nicotine consumption and diabetes, the observed trend of increases in the frequency of endothelial cell turnover and endothelial permeability to large molecules in the aorta of cholesterol-fed rats suggested that these changes may contribute to accelerated atherogenesis in hyperlipidemia. However, since rat is not a favored animal model for studies of diet-induced hyperlipidemia and atherosclerosis, further investigations using other animal models such as rabbit, are needed to verify these observations. |
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