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題 名 | 抗豆象綠豆對斜紋夜盜(Spodoptera litura Fabricius)存活與發育之影響=Effect of Bruchid-resistant Mungbean on Survival and Development of Spodoptera litura (F.) (Lepidoptera: Noctuidae) |
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作 者 | 耿璐; 許惠怡; 洪淑彬; | 書刊名 | 臺灣昆蟲 |
卷 期 | 24:3 2004.09[民93.09] |
頁 次 | 頁247-256 |
分類號 | 434.27 |
關鍵詞 | 抗豆象綠豆品系; 斜紋夜盜; 綠豆抗蟲物質; 抗蟲作用; Resistant mungbean; Spodoptera litura; Resistant substance; Resistance; |
語 文 | 中文(Chinese) |
中文摘要 | 自抗豆象綠豆中純化之綠豆防禦素VrD1(Vigna radiata Defensin 1)是首次被報導對豆象具有殺蟲作用之植物防禦素。為瞭解抗豆象綠豆是否對斜紋夜盜(Spodoptera litura Fabricius)亦具抗性作用,以作為探討綠豆抗蟲物質之殺蟲範圍及作用之基礎。本研究將不含綠豆粉、含5.26%感豆象品系(以下簡稱感蟲品系)的綠豆粉(VC1973A)及含5.26%抗豆象品系(以下簡稱抗蟲品系)之綠豆粉(VC6089A)等三種飼料分別餵食一齡第一天、第二天及二齡、四齡的斜紋夜盜幼蟲,觀察其生長、發育及死亡情形直到成蟲羽化。結果發現含感蟲或抗豆象綠豆粉處理組之飼料對一齡幼蟲影響最大,餵食含綠豆粉飼料一天後其死亡率已達100%。二齡及四齡幼蟲的處理,則除了殺蟲的效果外,在抑制生長及發育上也有顯著影響;二齡幼蟲處理組中,幼蟲期死亡率分別是抗豆象綠豆組>感蟲綠豆組>對照組,另外在各齡期發育日數及體重方面,也是以抗豆象綠豆組發育最遲緩,體重也最輕,感蟲綠豆組次之,對照組則發育最快,體重最重;四齡幼蟲處理組的結果與二齡處理組類似,不過因為二齡蟲組接受餵食含綠豆粉的飼料時間較長,因此幼蟲有藉延長發育期來加以補償,而達到相同體重的情形。從試驗結果推論,抗豆象綠豆中具有對斜紋夜盜有顯著抗蟲效果之抗蟲物質,在含抗豆象綠豆粉飼料中,VrD1濃度最高約僅0.0063%,因此以VrD1進行生物檢定方能釐清造成斜紋夜盜死亡及抑制發育之原因;另外綠豆抗蟲物質不只影響較小齡期的幼蟲,對較大齡期的幼蟲也有顯著影響。基於抗豆象綠豆試驗之結果,影響抗蟲作用之主要物質及其機制,值得進一步之比較研究,加以釐清。 |
英文摘要 | VrD1 (Vigna radiata Defensin 1) isolated from mungbean is the first plant defensin found to exhibit insecticidal effects. The aim of this study was to confirm the existence of a resistant substance in the mungbean and to characterize its resistance to Spodoptera litura F., an important pest in tropical areas. Hence the effect and mechanism of the resistant substance in the mungbean which is capable of being developed into a bioinsecticide or a transgenic crop could be evaluated. Artificial diets containing 5.26% of susceptible (VC1973A) or resistant mungbean (VC6089A) powders were provided to feed the first, second, and fourth instar larvae of S. litura, and the mortality, growth, and development of different stages of this insect were recorded. Larvae fed a diet without mungbean was served as a control group. Results showed that all the first instar larvae survived on the control diet, while diets containing susceptible or resistant mungbean powders caused 100% mortality to the first instar larvae. Feeding tests with the second and fourth instar larvae showed that the mortality of the immature stages significantly increased in the order of the control, susceptible, and resistant mungbean group. It was also observed that the diets containing the susceptible or resistant mungbean powder significantly extended the development period and decreased the fresh weight of the larvae and pupae. However, the second instar larva, which survived and consumed the diets with mungbean powder for a longer period, may have extended its development period in order to increase its body weight to a normal level in the final instar or pupal stages. These tests revealed that the resistant substance in the mungbean exhibits an insecticidal effect, and also has an inhibitory effect on the growth of S. litura larvae. Since the concentration of VrD1 in the diets with susceptible or resistant mungbeans was no more than 0.0063%, conducting bioassays with diets containing VrD1 is crucial for clarifying its resistance effect on S. litura in the future. |
本系統中英文摘要資訊取自各篇刊載內容。