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題名 | Ecologically Feasible Selection Strategy upon Indigenous Thiobacillus thiooxidans=生態上可行篩選本土性硫氧化硫化桿菌之策略 |
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作者 | 陳博彥; 侯邵毅; 劉宣良; 吳東峻; 林明樞; 鄭陽助; | 書刊名 | Journal of the Chinese Institute of Chemical Engineers |
卷期 | 35:5 2004.09[民93.09] |
頁次 | 頁587-593 |
分類號 | 460.02 |
關鍵詞 | 篩選; 硫氧化硫化桿菌; Thiobacillus thiooxidans; Ecologically stable selection; Acidophilic characteristics; Polyene antibiotics; |
語文 | 英文(English) |
中文摘要 | 本研究就實用觀點來提出一技術上可行且利於現地分離穩定特性之硫氧化硫化桿菌之策略。定量評估分析過程可明顯地指出純化過程之呈現結果為成功抑或失敗。酸化特性之持續提昇明顯表示令人肯定之篩選可行性及硫氧化硫化桿菌之穩定性。加入紐黴素並去除固體顆粒之步驟更可排除於稀釋純化過程可能存在之污染物種,因此可確保得到生態上具穩定特性之硫氧化硫化桿菌。硫酸形成速率之漸近收斂值更可強烈支持所得生態上純化之硫氧化硫化桿菌高純度特性,而於16S rDNA檢測鑑定之證據更再次確認比嗜酸性菌為硫氧化硫化桿菌,亦肯定吾人所提出連續純化策略之可行性。因此此菌株選種流程確實提供一理論上可行且符合經濟效益之策略來現地篩選嗜酸性硫氧化硫化桿菌以符合實際運用之需求。 |
英文摘要 | This study provides a technologically feasible strategy from a practical perspective for in-situ isolation of stable Thiobacillus thiooxidans. Quantitative assessment also clearly emphasizes the evolutionary outcome of enrichment appearing in dichotomous guise (i.e., success or failure; “yes/no”). Progressive increasing in acid formation capability presents the promising viability of selection scheme and stability of T. thiooxidans isolates. Incorporation of polyene antibiotics-nystatin and exclusion of solid particles efficiently evacuates possible contaminant species from soil-dilution samples to guarantee an ecologically stable T. thiooxidans to be resulted. Asymptotically convergent values of sulfuric acid formation rate strongly support high purity of ecologically enriched T. thiooxidans isolates. Further evidence in 16S rDNA detection clearly confirmed pure acidophilic isolate as T. thiooxidans, promising feasibility of our proposed scheme for serial isolation. This selection scheme provides a theoretically plausible and economically appropriate strategy to screen acidophilic T. thiooxidans for practical in situ applications. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。