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題 名 | Pressurized Liquid Extraction of Bioactive Compounds from Taiwan Pines=壓力流體萃取臺灣松樹的生物活性成分 |
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作 者 | 林正忠; 張傑明; 林孫基; | 書刊名 | Journal of the Chinese Institute of Chemical Engineers |
卷 期 | 35:4 2004.07[民93.07] |
頁 次 | 頁399-407 |
分類號 | 460.025 |
關鍵詞 | 壓力流體萃取; 松樹; 生物活性成分; 田口實驗技術; 回應曲面實驗法; 花青素; 抗氧化機制; Pine-needles; Pressurized liquid extraction; Taguchi method; Response surface methodology; Proanthocyanidins; Antioxidative activity; |
語 文 | 英文(English) |
中文摘要 | 本研究利用田口實驗技術(L[feda])結合回應曲面實驗法,以總產率(total yield)與前花青素(proanthocyanidin)含量為評估指標,探討壓力流體對臺灣數種松葉中前花青素萃取製程的最佳條件。實驗結果顯示,溫度效應難對產率有顯著影響,但相對亦可能造成生物活性成分的分解。在20% EtOH、140℃、750psig、1.5小時的最佳化製程條件下,總產率38%前花青素含量28.2mg/g與總黃酮含量29.75%,優於傳統50% EtOH溶劑萃取法的18.2%、13.60mg/g與14.77mg/g,可分別提升209%、207%與201%。在抗氧化活性評估項目中,臺灣二葉松萃出物的清除自由基活性(DPPH、超氧陰離子) 與抑制脂質過氧化的能力,分別為78.91%、81.73%、及76.72%;壓力流體萃取法有助活性成分總產率的提升。初步以反相樹脂,固相萃取純化二葉松葉的壓力流體萃出液,效果有限不如預期。松樹葉與皮活性成分的精製純化與抗氧化機制仍有待探討。 |
英文摘要 | An experiment combining the Taguchi methodology with the response surface methodology to optimize the Pressurized Liquid Extraction (PLE) of proanthocyanidins from Taiwan pines was conducted. A L[feda] orthogonal array was selected to investigate the total yield and the amount of proanthocyanidins. Experimental results showed that the extraction temperature most significantly affected the efficiency of extraction, but that a higher temperature also caused decomposition of bioactive components. Optimum conditions were identified as 20% EtOH, 140℃,750 psig, and 1.5h. The PLE total yield, proanthocyanidins content and total flavonoids were 2.09, 2.07, and 2.01 times greater than those obtained using a 50% EtOH conventional extraction method. Hot pressurized extraction has the advantages of low energy and high yield of bioactive compounds. The antioxidant activities of several extracts from two Taiwan pine-needles were individually evaluated for scavenging of DPPH free radicals and superoxide anion radicals, and for the inhibition of lipid peroxidation. The results revealed that both PLE M (Pinus morrisonicola) and PLE T (Pinus taiwanensis) extracted from two fresh pine needles scavenged 78.91% of DPPH free radicals. Air-dried pine needles also strongly inhibited superoxide anion radicals. The purification of four anthocyanidins from a PLE T extract was initially tested using HP-20 resin solid phase extraction and showed little enhancement. The isolation, purification and antioxidative mechanism of active components from pine needles need to be further investigated. |
本系統中英文摘要資訊取自各篇刊載內容。