頁籤選單縮合
題名 | A Study on the Refolding of Lysozyme Using Fed-Batch and Step-Addition Strategies=饋料式與間歇式復性法對溶菌□復性行為之研究 |
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作者 | 王勝仕; 張哲魁; 劉懷勝; | 書刊名 | Journal of the Chinese Institute of Chemical Engineers |
卷期 | 35:4 2004.07[民93.07] |
頁次 | 頁389-398 |
分類號 | 460.02 |
關鍵詞 | 饋料式復性法; 間歇式復性法; 溶菌□; 再摺疊程序; Lysozyme; Refolding; Fed-batch; Step-addition; |
語文 | 英文(English) |
中文摘要 | 利用基因重組技術生産蛋白質的過程中,蛋白質的再摺疊程式是將不具活性或不可溶解性之聚合體再恢復至原有生物活性的必經過程。直接稀釋法是復性程序中最簡單的方法,但如何提高其復性效率仍在持續研究中。本研究中,我們利用饋料式與間歇式不同的復性方法,以獨立地或結合地方式進行溶菌酶之復性。由實驗結果顯示,饋料式與間歇式復性法相較於稀釋法已有效地避免聚集體之生成及提高最終的操作濃度。此外,由於間歇式較饋料式提供更充足的摺疊時間間隔,故可獲得較高的活性回收率,而饋料式則具有高産率之優點。最後本研究中,結合饋料式與間歇式復性法之操作策略,可更有效率地提高其復性效率。 |
英文摘要 | The refolding step is essential for industrially produced protein aggregates to restore their biological activities. Various strategies, including the direct dilution method, have been utilized to enhance the performance of the refolding process; however, refinement and improvement on these methods are ongoing. We have used two different operating schemes, fed-batch and step-addition, independently and collaboratively to refold denatured lysozymes. Our research shows that the fed-batch and the step-addition schemes overcame the aggregation formation and favored the refolding step at higher feeding concentrations in comparison with the typical dilution method. Moreover, with relatively longer folding time intervals, the step-addition scheme provided higher activity recovery, while the fed-batch scheme was advantageous in terms of productivity. Finally, a combined scheme was proposed to optimize the refolding process, This work demonstrates the feasibility of applying fed-batch and/or step-addition operation schemes to the renaturation of enzyme activity. |
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