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頁籤選單縮合
題名 | Anti-Cancer Effect of Liriodenine on Human Lung Cancer Cells=Liriodenine對人類肺癌細胞抗癌效果之研究 |
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作者 | 張慧秋; 張芳榮; 吳永昌; 賴永勳; Chang, Hui-chiu; Chang, Fang-rong; Wu, Yang-chang; Lai, Yung-hsiung; |
期刊 | The Kaohsiung Journal of Medical Sciences |
出版日期 | 20040800 |
卷期 | 20:8 2004.08[民93.08] |
頁次 | 頁365-371 |
分類號 | 418.31 |
語文 | eng |
關鍵詞 | 細胞週期素; 細胞週期素依賴型蛋白激酶; 細胞凋亡; Liriodenine; Cyclin; Cyclin-dependent kinase; Poly(ADP-ribose) polymerase; Apoptosis; |
中文摘要 | 我們先的研究發現多種本物中所含的生物鹼liriodenine可毒殺多種人類癌細胞,本研究中我們探討liriodenine對人類肺癌細胞生長與存活的影響及其作用之機制,我們發現liriodenine可明顯抑制A549肺癌細胞之生長,流式細胞儀分析顯示細胞週期進行被阻斷在G2/M期,同時LIRIODENINE處理降低G1週期素(D1)的表現,但G2週期素(B1)則明顯增加。試管內蛋白激?活性分析發現cyclin B1/CDK1活性被liriodenine顯著抑制。更重要的,liriodenine會誘發A549細胞凋亡,當細胞增養於低濃度血清時,其毒殺作用更明顯。綜言之,我們結果顯示liriodenine對肺癌細胞具有抑制生長與誘發凋亡之作用。 |
英文摘要 | Our previous work demonstrated that liriodenine, an isoquinoline alkaloid isolated from plat species of many genera, exhibits a potent cytotoxic effect on vairous types of human cancer cells. In this study, we investigated the effect of liriodenine on the growth and viability of human lung cancer cells and addressed the underlying mechanism of action. We found that lirodenine suppressed proliferation of A549 human lung adenocarcinoma cells in a does-and time-dependent manner. Flow cytometric analysis showed that liriodenine blocked cell cycle progression at the G2/M phase. Induction of G2/M arrest by liriodenine was accmpanied by reduction of G1 cyclin (D1) and accumulation of G2 cyclin (B1). Invitro kinase activity assay demonstrated that the enzymatic activity of the cyclin B1/cyclin-dependent kinase 1complex was reduced in liriodenine-treated cells. The apoptosis-inducing actiity of liriodenine was more apparent when cells were cultured under serum-free conditions. Our results indicate that liriodenine exerts potent anti-proliferative and apoptosis-inducing effects on human lung cancer cells. |
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