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頁籤選單縮合
題名 | Gel Electrophoresis in Polydimethylsiloxane for DNA Purification=矽膠材質DNA純化晶片 |
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作者 | 林建明; 胡文聰; 張裕釧; 林畢修平; Lin, Cheng Ming; Wo, Andrew; Jang, Yuh-chuahn; Lin, P. Hsiu-ping; |
期刊 | 國立臺灣大學工程學刊 |
出版日期 | 20040600 |
卷期 | 91 2004.06[民93.06] |
頁次 | 頁75-79 |
分類號 | 440.34 |
語文 | eng |
關鍵詞 | DNA純化; 電泳; 洋菜膠; PDMS; DNA purification; Electrophoresis; Agarose gel; |
中文摘要 | 本文目標朝向微小DNA純化系統進行,利用微機電系統和生物技術,可將流體元件微小化,可以達到實驗室晶片(lab-on-a-chip)的理想目標,我們所使用的軟性材料為polydimethylsiloxane (PDMS) 彈性材料,可以快速製作微流道、微混和器和微過濾器等元件製成微流體晶片的初步原形。在微流道內注入洋蔡膠體當作分子篩子。為提供高壓電源加速分離DNA和雜質,利用電晶體 (bipolar junction transistor) 電路產生一高壓電源;實驗結果證明,在微膠體電泳實驗裡,較小的分子能通過膠體分子篩;螢光球因本身有較大的尺寸(0.9m )而被膠體阻擋,而單股的螢光DNA分子可以從混合液中分離出來,本實驗設計的微流體晶片具有分離且有純化DNA的能力。 |
英文摘要 | This paper reports progress towards DNA purification in a microfabricated device. In order to achieve the lab-on-a-chip, components – microchannel, micromixer, microfilter, and electrophoresis module are fabricated using polymeric material polydimethylsiloxane (PDMS) for fast prototyping. The microchannel is injected with agarose gel, which acts as a sieve for bio-samples. To expedite the electrophoretic process during differentiating DNA and impurities as they pass through the gel, a bipolar junction transistor high-voltage device is fabricated. Results show that fluorescent micro-spheres (0.9m in diameter) are successfully separated from single strand DNA, which are collected in the micro-channel outlet. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。