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題名 | Morphological Alterations in the Trachea of Capsaicin-pretreated Rat During Postnatal Development=經辣椒素處理大白鼠氣管出生後發育的形態學變化 |
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作者 | 游祥明; 林光華; Yu, Shang-ming; Lin, Kwan-hwa; |
期刊 | 動物研究學刊 |
出版日期 | 20020100 |
卷期 | 41:1 2002.01[民91.01] |
頁次 | 頁13-22 |
分類號 | 414.82 |
語文 | eng |
關鍵詞 | 辣椒素; 免疫細胞化學; 出生後發育; P物質; 氣管; Capsaicin; Immunocytochemistry; Postnatal development; Substance P; Trachea; |
中文摘要 | 本研究的目的在探討初生大白鼠經辣椒素處後的氣管內形態學和黏膜厚度的變化。十七隻經辣椒素處理和十五隻對照組的老鼠,採用三聚甲醛-離氨酸-過碘酸鹽和鋨酸固的樹酯切片製作,並用光學顯微鏡來觀察。結果顯示,大白鼠氣管上皮的平均橫斷面厚度,在經辣椒素處理後的第一周為20.40±0.48μm與同期對照組的21.80±0.80μm並無顯著性的差異。氣管上皮的平均橫斷面厚度經辣椒素處理後的第二周實驗組為18.81±0.39μm與第一周實驗組仍無顯著性的差異,但與第二周對照組的40.05±1.80μm,卻有顯著性的差異(p<0.05)。再者,氣管上皮的平均極斷面厚度在經辣椒素處理後的第一個月為41.24±1.20μm,與同期對照組24.79±0.54μm相較也具有顯著性的差異(p<0.05)。在光學顯微鏡下,經辣椒素處理後在第一周時主要紟徵為顯著大空泡的形成,到第二周時有許多小空泡存在。另一顯著特徵為第二周時杯狀細胞的頂部具有圓徵外突的細胞質略為增加,到第二周時,卻更顯著地增加。在對照組,氣管上皮的P物質免疫細胞化學活性,在第一周時幾乎無法辨識,在第二周時免疫細胞化學活性略為增加,而到第一個月時P 物質免疫細胞化學活性卻顯著地增加。然而,在實驗組氣管上皮的P物質免疫細胞化學活性,在第周、第二周和第一個月時幾乎無法辨識其存在。目前的實驗結果指出,初生時辣椒素處理可造成發育中九管上皮厚度的減小。經辣椒素處理後,含有P物質免疫細胞化學活性的彈性纖維被抑制,可能與辣椒素排空P物質的效應有關。再?,杯狀細胞的頂部細胞質圓形外突的形成,可能系為黏液分泌與清除的機制受到辣椒素抑制的緣故。 |
英文摘要 | The purpose of this study was to investigate morphological changes of the mucosa layer of the trachea in rats pretreated with capsaicin neonatally. Parafomaldehyd-lysine-periodate- and osmuin-fixed plastic sections were prepared from 17 capsaicin pretreated rats and 15 controls and studied at the light microscopic level. The results indicate that no significant differences occurred in the mean cross-sectional epithelial thickness of the tracheal mucosa between 1 wk after (20.40±0.48μm) and sham treatment (21.80±0.80μm). Cross-sectional capsaicin treatment, but the epithelial thickness (18.81±0.39μm) continued to show on significant difference through 2 wk after neonatal (40.05±1.80μm). Furthermore, cross-sectional epithelial thickness also significantly (p<0.05) increased at 1 mo after sham treatment (41.24±1.20μm) compared to that with neonatal capsaicin treatment (24.79±0.54μm). At the light microscopic level, large vacuoles were strickingly prominent in the tracheal epithelium at 1wk after neonatal capsaicin treatment, as were many small vacuoles at 2wk. Another striking change was that aplical cytoplasmic blebbing of goblet cells had increased moderately at wk after neonatal capsaich treatment and had increased markedly at 1 mo. Substance P-like immunoreactivity (IR) was not discernible at 1wk after sham or capsaicin treatment. Substance P-like IR was slightly immunolabeled at 12wk and increased markedly at 1 mo after sham treatment. In contrast, substance P-like IR was not immunolabeled at either 2wk or 1 mo after neonatal capsaicin treatment. The present study suggests that neonatal capsaicin treatment result in epidermal thinning of the trachea during postnatal development. The blockade of the elastic fibers containing results in epidermal thinning of the trachea during postnatal development. The blockade of the elastic fibers containing substance P-like IR implies of the elastic fibers in the histologic response to capsaicin’s effect on the depletion of substance P Moreover, fmrmation of capical cytoplasmic blebbing of goblet cells indicates hindrance by capsaicin of mucus secretion and clearance. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。