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頁籤選單縮合
題 名 | 以轉錄層次探討藍綠藻和葉綠體在演化上的關係=The Evolution between Cyanobacterium and Chloroplast in Transcriptional Level |
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作 者 | 柴御清; 陳伯中; 陳良築; | 書刊名 | 中州學報 |
卷 期 | 19 2004.06[民93.06] |
頁 次 | 頁165-174 |
分類號 | 371.38 |
關鍵詞 | 內生性假說; 演化; 葉綠體; 藍綠藻; 轉錄; Chloroplast; Cyanobacteria; Endosymbiosis; Evolution; Transcription; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究抽取具有生體外轉錄活性之菠菜葉綠體及魚腥藻兩種蛋白粗抽萃取液,並配合菠菜葉綠體rbc L基因及魚腥藻rbc LS基因起動子進行反應。結果發現,魚腥藻蛋白粗抽萃取液均可辨認菠菜葉綠體rbc L基因及魚腥藻rbc LS基因起動子,分別轉錄出預期約230 n.t.及780 n.t.大小之專一性RNA產物;菠菜葉綠體蛋白粗抽萃取液則只能辨認本身之菠菜葉綠體rbc L基因起動子,無法辨認魚腥藻rbc LS基因起動子;兩種蛋白粗抽萃取液混合,進行辨認魚腥藻rbc LS基因起動子反應時,其轉錄效率和只含單一魚腥藻蛋白粗抽萃取液比較,有加成的情形;並利用BSA做為結構性蛋白的角色,發現此加成情形與兩蛋白粗抽萃取液內酵素交互作用有關。 根據以上實驗結果推論,在葉綠體演化時失去某些蛋白,而使葉綠體無法辨認魚腥藻的基因起動子。另一方面,魚腥藻之演化層次較低,故此蛋白仍保留,可協助魚腥藻辨認基因起動子,所以當兩種蛋白混合液進行轉錄反應時,葉綠體就由魚腥藻蛋白粗抽萃取液中,獲得反應加成效果。此現象將可解釋藍綠藻與葉綠體之間關連性,並做為支持內生性假說的証據之一。 |
英文摘要 | There are two active RNA polymerase crude extracts from the spinach chloroplast and Anabaena CH1 strain were match with the spinach chloroplast rbc L gene and Anabaena CH1 rbc LS gene promoters in vitro transcription assay. While the CH1 RNA polymerase extract could recognize both the promoters of Anabaena rbc LS gene and spinach chloroplast rbc L gene, and the chloroplast RNA polymerase extract could only recognize the chloroplast promoter but not the Anabaena promoter. However, adding spinach chloroplast extract to the transcription reaction with CH1 RNA polymerase extract will significantly enhance the transcription efficiency. This phenomenon implied that some kinds of the interaction that could positively regulate transcription reaction may exist between two enzyme extracts. According to this result, we are assumption that the plant chloroplast was lost some transcriptional factors that could not recognize the Anabaena gene promoter during the evolutional pathway. Because of the cyanobacteria is primordial better than chloroplast in photosynthesis, maybe the transcriptional factor is still reserves in Anabaena and it can recognize both promoters. The chloroplast extract will recognize the Anabaena gene promoter and enhance the transcription efficiency when two kinds of RNA polymerase extracted mixtures in the same assay. This phenomenon will explain the relationship between cyanobacteria and chloroplast and can be used to support of the hypothesis of endosymbiosis. |
本系統中英文摘要資訊取自各篇刊載內容。