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題 名 | The Effects of Vero Cell Co-Culture on Human Zygotes Resulting from in Vitro Fertilization and Oocytes Following Subzonal Insemination=綠猴腎細胞共同培養系統對人類試管受精合子及卵子透明帶下顯微注射受精卵子之作用 |
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作 者 | 賴英明; 張明揚; 張甫行; 李奇龍; 李俊德; 黃泓淵; 張旭陽; 王美莉; 詹平如; 宋永魁; | 書刊名 | 長庚醫學 |
卷 期 | 19:3 1996.09[民85.09] |
頁 次 | 頁203-210 |
分類號 | 417.125 |
關鍵詞 | 共同培養; 試管受精; 透明帶下受精; Co-culture; In vitro fertilization; Subzonal insemination; |
語 文 | 英文(English) |
中文摘要 | 在傳統的試管嬰兒(IVF)培養系統中,人類胚胎不但發育得比在輸卵管中慢,而且長到四至八個細胞期時就會停頓不長,然而提早植入子宮的結果,卻都因為子宮內膜尚未完成準備而導致胚胎著床率及懷孕率無法突破瓶頸。我們在動物實驗中發現綠猴腎細胞(Vero cell)共同培養系統對人類胚胎發育有幫助的效果,因此進行本實驗以探討共同培養系統對人類卵子及胚胎的影響。本研究分為兩部份,第一部分是將1695個體外受精(IVF)之雙原核期胚胎分成兩組培養,24小時後記錄胚胎平均胚細胞數及胚胎品質優劣,然後再植入子宮。其中以Vero cell共同培養者有955個,培養24小時後平均胚細胞數為4.01 [] 1.32,幼質胚胎率為11.5%;而以傳統培養法約有740個,其平均胚細胞數為3.86 [] 1.45,劣質胚胎率為19.9%;兩項的比較均為有意義的差別(p<0.05)。本研究的第二部分是將卵子以透明帶下精蟲注射(SUZI)後,馬上分成上述兩組進行培養,培養48小時後記錄胚胎平均胚細胞數及胚胎品質然後再植入子宮。 其中共同培養者有66個,其平均胚細胞數為3.33 [] 1.34,劣質胚胎率為9%;而傳統培養法平均胚細胞數為3.11 [] 1.34,劣質胚胎率為27%;其中劣質胚胎率的比較也是有意義的差別(p<0.05)。 本研究的結論是Vero cell共同培養系統,無論對一般試管嬰兒(IVF)胚胎或卵子透明帶下精蟲注射(SUZI)胚胎的發育,都有幫助的作用。雖然作過卵子透明帶下精蟲注射(SUZI)的胚胎,因為透明帶的創傷而發育比一般試管嬰兒(IVF)胚胎慢,平均胚細胞數少,但是劣質胚細胞率的改善則更為明顯(9%對27%)。由於本研究只是對人類胚胎作短期的培養,共同培養系統所產生的效果有限,因此必須作更長期的培養才能觀察到共同培養與傳統培養法兩者在懷孕以及著床率方面,更加明顯的差別。 |
英文摘要 | A variety of co-culture systems have been devised to enhance the human embryo development in vitro. Vero cells were selected because they can be highly controlled and are easy to handle. To evaluate the embryotrophic effects of Vero cell monolayers, when they were co-cultured with human in vitro fertilized zygotes or subzonal inseminated oocytes. Total 1695 two-pronuclear embryos resulting from in vitro fertilization were cultured with Vero cells or medium alone for 24 fours. Similarly, sixty-six two-pronu-clear embryos resulted from subzonal insertion of sperm (SUZI) with co-culture starting immediately following SUZI were compared with fifty-two two-pronuclear embryos resulted from SUZI, without co-culture. The numbers of blastomere and morphology of embryos were compared between the co-culture group and control group using student's t-test. Cell numbers in each embryo were greater in the IVF/co-culture group than in the control group (4.01 [] 1.32 vs. 3.86 [] 4.45, p<0.05). The rates of poor quality embryo with major fragmentation were lower in the co-culture group than in the control group (11.5% vs. 19.9%, p<0.001, for IVF embryos; 9% vs. 27%, p<0.005, for SUZI oocytes). Co-culture SUZI oocytes on Vero cells prior to fertilization did not positively influence embryo cleavage, but improved embryo quality. We conclude that Vero cells can enhance human embryo development; however, the period for one-day or two-day co-culture is too short to provide a maximal support. Short term co-culture did not increas implantation rates. Immediate co-culture following SUZI might somewhat rescue the microinseminated oocytes. However, a longer duration of co-culture is necessary to exert the maximal effects on embryo development and implantation. |
本系統中英文摘要資訊取自各篇刊載內容。