查詢結果分析
來源資料
相關文獻
- Establishment of cDNA Microarray Analysis at the Genomic Medicine Research Core Laboratory (GMRCL) of Chang Gung Memorial Hospital
- 提升服務有形程度的行銷原則--模式建構與實驗研究
- The Optimal Hydrodynamic Design of a Three-Phase Draft-Tube Fluidized Bed with an Orthogonal Array
- 企業教育訓練成效評估模式之實驗研究--以燁隆鋼鐵公司為例
- 應用因子實驗設計法改善工業用油配方
- 社區民眾健康促進自我導向學習經驗研究--以學童母親自我導向學習急救知能為例
- 應用灰色GM(1,N)模型及田口實驗法於塑膠射出成型加工上之研究
- 電腦輔助視覺傳達設計--平面構成轉換的實驗設計
- 三線包縫針步爆破強力統計分析
- 藉由電腦輔助,執行高品質,低成本之公差設計
頁籤選單縮合
題名 | Establishment of cDNA Microarray Analysis at the Genomic Medicine Research Core Laboratory (GMRCL) of Chang Gung Memorial Hospital=長庚紀念醫院基因體醫學核心實驗室cDNA微陣列分析系統之建立 |
---|---|
作者姓名(中文) | 王子豪; 李御賢; 陳恩賜; 龔維祥; 陳龍坤; 薛丁瑋; 魏敏莉; 王馨世; 李英雄; | 書刊名 | 長庚醫學 |
卷期 | 27:4 2004.04[民93.04] |
頁次 | 頁243-260 |
分類號 | 364.6 |
關鍵詞 | DNA微陣列技術; 實驗設計; 基因體醫學核心實驗室; 標準操作程序; 品質確保動作; 微陣列實驗最基本資訊; DNA microarray technology; Design of experiments; Genomic medicine research core laboratory; GMRCL; Standard operating procedure; Quality assurance; Minimal information about a microarray experiment; MIAME; |
語文 | 英文(English) |
中文摘要 | 背景:隨著分子及資訊生物學之進步,人類已發展出強大且具高產能之分析因因表現的方法,開啟了基因體醫學研究之新紀元。DNA微陣例技術已被積極地整合至基礎生物醫學研究,並將成為許多臨床疾病之分子監視工具。 方法:長庚紀念醫院基因體醫學核心實驗室為了能提供院校內科各科系研究人員利用DNA微陣列技術來探討基因體醫學,本核心實驗至建構後之首要工作是建立各項儀器之標準操作程序,並確定每個執行步驟之品質。 結果:第一年已完成測試,這些執行步驟包括:細菌庫之複製、人類DNA株之放大,每個DNA株之詮釋、製作cDNA微陣列晶片、RNA質與量之檢測、目標檢體之螢光標定、競爭雜合反應、晶片之掃瞄、資料分析、及微陣例後結果之確定。文中敘述基因體醫學核心實驗室所使用之方法步驟,並討論其必要性。 結論:本文報告長庚紀念醫院基因體醫學核心實驗室所使用之方法步驟,與利用合乎微陣列實驗最基本資訊(MIAME)規範來檢討之cDNA微陣列結果。這些資訊不但對使用本核心實驗室之研究者有所幫忙,對將在其他機構建立自己的DNA微陣例系統之研究者也有助益。 |
英文摘要 | Background: Advances in molecular and computational biology have led to the development of powerful, high-throughput methods for analysis of differential gene expression, which are opening up new opportunities in genomic medicine. DNA microarray technology had been enthusiastically integrated into basic biomedical research and will eventually become a molecular monitoring tool for various clinical courses. Methods: As a core research facility of Chang Gung University (CGU) and Chang Gung Memorial Hospital (CGMH), the Genomic Medicine Research Core Laboratory (GMRCL) welcomes investigators from every discipline to employ DNA micorarray technology in the quest for knowledge of genomic medicine. The first tasks for GMRCL are to optimize the standard operating procedures (SOP) for each instrument and to assure the quality of every procedure. Results: During the first year after the establishment of the GMRCL at the CGMH, we tested and adopted procedures that were satisfactory for our purposes. These procedures included: replication of bacterial stocks, amplification of human DNA clones, annotation of each DNA clone, production of cDNA microarrays, validation of RNA quality and quantity, labeling of target speciments, competitive hybridization, scanning of slides, data analysis, and post-microarray validiation of results. We present a summariazation of the materials and procedures used at the GMRCL and discuss the reasons for using them. Conclusions: The information about the cDNA microarray analysis system at the GMRCL is compliant with the minimal information about a microarray experiment (MIAME) format. The information may be useful to both the investigators who are using this core facility and researchers at other institutes, who will establish their own in-house cDNA micorarray systems. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。