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頁籤選單縮合
題 名 | Determination of Major Carotenoids in Human Serum by Liquid Chromatography=以液相層析法測定人體血液中的主要類胡蘿蔔素 |
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作 者 | 曾明淑; 楊駱菲莉; 王果行; 陳炳輝; | 書刊名 | 藥物食品分析 |
卷 期 | 12:1 2004.03[民93.03] |
頁 次 | 頁79-83+101 |
分類號 | 414.7 |
關鍵詞 | 高效率液相層析法; 人體血液; 類胡蘿蔔素; Carotenoids; Human serum; HPLC; |
語 文 | 英文(English) |
中文摘要 | 一種簡單快速的高效率液相層析法已被發展出,以測定人體血液中的主要類胡蘿蔔素。使用C18管柱配合等比動相甲醇/二氯甲烷 (95:5,v/v) 及樣品溶劑甲醇/二氯甲烷 (45:55,v/v) ,偵測波長476nm和流速0.8mL/min,可於20分鐘內分離出葉黃素、β胡蘿蔔素及番茄紅素,且回收率分別為92,90及87%。使用此方法分析由老人安養中心收集的62個血液樣品,結果顯示大部分血液樣品具有最高量的β一胡蘿蔔素,次為葉黃素及番茄紅素。 |
英文摘要 | A simple and rapid high performance liquid chromatographic (HPLC) method was developed to determine the major carotenoids in human serum. Lutein, b-carotene and lycopene were separated within 20 min using a C18 column and a mobile phase of methanol/methylene chloride (95:5, v/v) with sample solvent methanol/methylene chloride (45:55, v/v), detection at 476 nm and flow rate at 0.8 mL/min. A high recovery was achieved with this method, which amounted to 92, 90 and 87% for lutein, b-carotene and lycopene, respectively, while the detection limit was 5.0, 10.0 and 4.0 ng/mL. This method was applied to determine the major carotenoids in human serum samples collected from an elderly care center. A total of 62 samples were measured, and in most cases,b-carotene (17.5-1105.7 ng/mL) was present in the highest amount, followed by lutein (10.6-182.3 ng/mL) and lycopene (0-402 ng/mL). |
本系統中英文摘要資訊取自各篇刊載內容。