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| 題 名 | Keratinocyte-fibroblast Cocultures on A Bi-layered Gelatin Scaffold for Skin Equivalent Tissue Engineering=以角質細胞及真皮纖維母細胞共養於雙層明膠薄膜作為類皮膚組織工程之研究 |
|---|---|
| 作 者 | 王子威; 黃怡超; 孫瑞昇; 林峰輝; | 書刊名 | Journal of Medical and Biological Engineering |
| 卷 期 | 23:4 2003.12[民92.12] |
| 頁 次 | 頁213-219 |
| 分類號 | 398.1 |
| 關鍵詞 | 海綿明膠; 葡萄氨聚糖; 角質細胞; 真皮纖維母細胞; 類皮膚; Sponge gelatin; Glycosaminoglycan; Keratinocyte; Dermal fibroblast; Skin equivalent; |
| 語 文 | 英文(English) |
| 中文摘要 | 皮膚是由真皮層及表皮層所組成,為了以組織工程的方法發展一個類皮膚,藉以幫助大片燒燙傷病人皮膚修復,本研究室開發出雙層的海綿明膠薄膜,材料中加入透明質酸及軟骨素以模仿皮膚的成份,上層在-20℃冷凍乾燥下形成較小的孔隙,以培養角質細胞發育成表皮層。下層在-196℃冷凍乾燥下形成較大的孔隙,以培養真皮纖維母細胞發育成真皮層。在液氣面培養21天後,類皮膚以光學顯微鏡及電子顯微鏡觀察,同時偵測細胞激素含量,觀察細胞活性。 |
| 英文摘要 | The skin is composed of three parts: epidermis, dermis and subcutaneous fatty layers. In order to develop a biomimetic material for more realistic tissue regeneration, we fabricated a bi-layered gelatin sponge with different pore sizes by freeze-drying at different temperatures -20℃ and -196℃, respectively. A rete ridges-like topographic microporous structure, which provided the paracrine crosstalk in epithelial-mesenchymal interactions similar to the basement membrane in epidermal-dermal junction, was formed between these two compartments. Chrondroitin-6-Sulphate (C-6-S) and hyaluronic acid (HA) were incorporated within the gelatin scaffold to create an appropriate microenvironment for cell proliferation and migration. The mechanical strength of gelatin sponges was improved by cross-linking of gelatin with 1-ethyl-3 (3-dimethyl aminopropryl) carbodiimide (EDC). The lattice structure and pore sizes were evaluated by SEM to confirm the interconnected porous structures retained. We chose a dynamic spinner flask seeding method for more evenly distribution followed by a culture system in the air-liquid interface and cultured for 21 days. Differentiation and phenotypic expression of keratinocytes were investigated by histological analysis. In this study, we found a multiple epidermis-like layers constructed by cultured keratinocytes. It is suggested that the bi-layered scaffolds have the potential to be used as skin equivalents for the application in burn patients. In the future, the in vivo animal model tests will be evaluated. |
本系統中英文摘要資訊取自各篇刊載內容。