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題名 | Differential Effects of Endothelin-1 on Calcium Mobility in Cultured Porcine Pigment Epithelial Cells of Iris and Retina=內皮素-1對培養的豬虹膜及視網膜色素上皮細胞內鈣離子的移動性的效果 |
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作者姓名(中文) | 吳國揚; 洪秀貞; 馮濟敏; 王惠珠; | 書刊名 | 臺灣醫學會雜誌 |
卷期 | 102:7 2003.07[民92.07] |
頁次 | 頁465-473 |
分類號 | 416.746 |
關鍵詞 | 豬虹膜; 視網膜; 內皮素-1; 鈣離子; Calcium signaling; Endothelin-1; Pigment epithelium of the eye; Receptors; Endothelin; |
語文 | 英文(English) |
英文摘要 | Background and Purpose: Photoreceptor function depends on an intact retina pigment epithelial (RPE) cell layer. Transplantation of iris pigment epithelial (IPE) cells to the subretinal space offers a potential alternative to RPE transplantation in patients with severe retinal diseases such as macular degeneration. Whether the functions of IPE and PRE are similar or different is not well established. The purpose of this study was to identify the characteristics of IPE cells and RPE cells by detecting the effects of endothelin-1 (ET-1) on intracellular free Ca2+ ([Ca2+]i) mobility in these cells. Methods: Iris and retina pigment epithelial cells were cultured from porcine eyeballs. After the cells were loaded with fura-2/AM, fluorescence was monitored with a fluoresent spectrophotometer by continuously recording excitation signals at 340 nm and 380 nm and emission signals at 510 nm. Results: IPE and RPE cells induced a significant increase in [Ca2+]i under exposure to 10-7, 10-8, and 10-9mol/L ET-1 in iris but not in retina pigment epithelial cells. In Ca2+-free buffer, pretreatment of the IPE cells with 10-7 mol/L ET-1 partially inhibited thapsigargin-induced [Ca2+] increase and carbonylcyanide m-chlorophenylhydrazone (CCCP)-induced Ca2+ release. Similarly, pretreatment of the IPE cells with thapsigargin or CCCP also partially inhibited ET-l-induced[Ca2+]i increase. The [Ca2+]i release induced by 10-7 mol/L ET-1 was not inhibited by the phospholipase C inhibitor U73122. In ca2+-containing buffer, the ETA receptor antagonist BQ123 and ETB receptor antagonist BQ788 partially inhibited 10-7mol/L ET-1-induced [Ca2+]i increase in IPE cells. However, BQ123 partially inhibited the 10-7mol/L ET-1-induced [Ca2+] i increase in RPE cells. Nifedipine partially inhibited 10-7mol/L ET-1-induced [Ca2+]i increase in both types of pigment cells. Conclusions: These results suggest that iris and retina pigment epithelial cells posses distinguishing characteristics because the ET-1-induced [Ca2+]i increases in these 2 types of pigment epithelial cells are regulated by distinct mechanisms. |
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