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| 題 名 | High-Resolution Optical Doppler Tomography for in Vitro and in Vivo Fluid Flow Dynamics=高解析光學式都卜勒斷層攝影術之流體動力之偵測 |
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| 作 者 | 張承仁; 侯光華; | 書刊名 | 長庚醫學 |
| 卷 期 | 26:6 2003.06[民92.06] |
| 頁 次 | 頁403-411 |
| 分類號 | 416.14 |
| 關鍵詞 | 光學式都卜勒斷層攝影術; 雷射都卜勒流量計; 光學式相干斷層攝影術; 顯微性血管; Optical Doppler tomography; Laser Doppler flowmetry; Optical coherence tomography; Microvasculature; |
| 語 文 | 英文(English) |
| 中文摘要 | 背景:本研究之目標在於運用非侵入性高解析度斷層攝影術來偵測高散射性生體組織之流體速度及顯微血管結構。 方法:光學式都卜勒斷層攝影術乃是結合雷射都卜勒流量計與光學式相干斷層攝影術之高解析技術,以得到對高散射性生體組織結構斷層攝影之影像及血流流速之偵測。為此,吾人運用光學超音波斷層攝影術於試管內混濁液體(含多聚脂顯微顆粒)及液狀脂肪在真角管徑內流速之偵測。同時也在活體動物模型,裸鼠耳朵,偵測其皮膚之血流。 結果:在混濁液於環管內流速偵測得知管徑中央之顆粒流速較管徑旁之顆粒流速為快,同時也在液狀脂肪的流速偵測得到類似的結果。而在動物模型,靜脈血管直徑於40至70微米者可偵測到其影像結構及流速。而動脈則是在25微米便可。 結論:光學式都卜勒斷層攝影術的運用將有助於吾人偵測特定部位高散射性生體組織之結構影像及血流速度。 |
| 英文摘要 | Background: The objective of our research was to use a noninvasive tomographic imaging technique with high spatial resolution (2-15 []m) to characterize and monitor fluid flow and the microvasculature in highly scattering biological tissues at user-specified discrete locations. Methods: The technique of optical Doppler tomography (ODT) combines laser Doppler flowmetry (LDF) with optical coherence tomography to obtain high-resolution tomographic velocity and structural images of static and moving constituents in highly scattering biological tissues. We present ODT structural and velocity images using in vitro turbid samples of a circular conduit infused with a suspension of polymer microsopheres. At a thin rectangular cross-section of the conduit, the Intralipid flow was measured. Blood flow velocity was measured in vivo in the ear of rodent skin. Results: In first model, the ODT velocity images demonstrated beads near the center of the conduit moving faster than those near the circular wall. In the second model, the ODT velocity images indicated that laminar flow was fastest along the central axis of the conduit. Blood flow in 2 small veins with diameters of 70 and 40 []m, respectively, and an artery with diameter of 25 []m, was clearly identified in a rodent model. Conclusion: In our preliminary in vitro and in vivo studies on turbid samples and model vasculatures, we determined that the application of DOT to charcterize and image blood flow with high spatial resolution at discrete user-specified locations in highly scattering biological tissues is feasible. |
本系統中英文摘要資訊取自各篇刊載內容。