查詢結果分析
來源資料
相關文獻
- 脆鐵線蕨孢子無菌撒播繁殖體系之建立
- 試管內乙烯與激勃素含量對脆鐵線蕨原葉體發育之影響
- 美洲水蕨原葉體和幼孢子體的生長及發育之研究
- 臺灣南部常見蕨類之孢子體細部構造及其原葉體發育過程之研究
- Exine Development in Borago (Boraginaceae) (1)--Microspore Tetrad Period
- Ultrastructural Study of X-ray Irradiated Spores of Equisetum Arvense L.
- Ultrastructural Study of Wall Ontogeny during Zygosporogenesis in Rhizopus Stolonifer (Mucoraceae), an Amended Model
- 臺灣地區麻雀糞便內寄生蟲種類之分析
- 臺灣原生蕨類孢子微細結構之觀察與繁殖技術之研究
- Exine Development in Borago (Boraginaceae) 2.Free Microspore Stages
頁籤選單縮合
題名 | 脆鐵線蕨孢子無菌撒播繁殖體系之建立=Partial Tissue Culture System for the Mass Propagation of Adiantum tenerum "Scutum Roseum" from Spores |
---|---|
作者姓名(中文) | 高秀雲; 葉德銘; | 書刊名 | 臺灣林業科學 |
卷期 | 18:1 2003.03[民92.03] |
頁次 | 頁33-42 |
分類號 | 378.13 |
關鍵詞 | 脆鐵線蕨; 孢子; 原葉體; 無菌繁殖; Adiantum tenerum Scutum Roseum; Spore; Prothallium; In vitro propagation; |
語文 | 中文(Chinese) |
中文摘要 | 脆鐵線蕨(Adiantum tenerum �Scutum Roseum?孢子播於無土介質及試管內,比較其原葉體生長及發育。孢子播於泥炭苔、蛭石與珍珠砂之無土混合介質下,原葉體長度約1.2 mm、寬度約1.3 mm以上時,即有藏精器的分化;原葉體長度約2.0 mm、寬度約2.5 mm,方觀察到藏卵器之形成。將孢子播於試管內,經增殖而來的原葉體僅分裂數個細胞後即有藏精器的形成。且於無菌撒播20 d後,自翼片下側近假根處,開始有原葉體的增殖與鮮重之增加。於60 d後,原葉體的鮮重即無明顯增加,而葉綠素含量下降。適當的繼代時期為無菌撒播後50-60 d,於第一次繼代6-8 wk後需再行第二次繼代,以使原葉體大量增生。若將無菌撒播60 d之原葉體團,以全量強生氏營養液浸泡5 sec後,再置於無土介質中,其孢子體形成速率,較前人所推薦之瞎S為佳。本研究建立以部分組織培養方式,可兼顧原葉體培育、增殖並可獲得更多的孢子體。 |
英文摘要 | Comparative growth and development of gametophyes and sporophytes were determined in Adiantum tenerum �Scutum Roseum?sown in a soilless mix and cultured in vitro. Prothallia in the soilless mix were competent to initiate antheridia provided that these prothallia had reached a critical size at a length of >1.2 mm and a width of >1.3 mm, while archegonia were observed with prothallia at a length of >2.0 mm and a width of >2.5 mm. In contrast, antheridia were observed in young adventitious prothallia cultured in vitro to have only a few cells. Young adventitious prothallia were differentiated from the lower region of wing cells of the prothallia after spores had been sown in vitro for 20 d. After sowing for 60 d, the fresh weight of prothallia had increased due to adventitious outgrowths. The fresh weight of prothallia had increased slightly, and the chlorophyll content had begun to decrease after having been sown in vitro for 60 d. The first optimum in vitro transfer duration was approximately 50-60 d after sowing, and the second subculture was 6-8 wk after the former transfer. Prothallia soaked with Johnson�s solution for 5 sec before transfer from in vitro culture to the soilless mix produced faster sporophyte formation than those soaked with 1/2 MS as previously recommended. The established partial tissue culture system could facilitate both the mass propagation of prothallia and production of a greater number of sporophytes. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。