頁籤選單縮合
題 名 | Fabrication of Piezoelectric Biochips with Self-Assembled Alkanethiol Layer and Hydrocoating=結合自排性長鏈硫醇膜與水膠塗覆法製備壓電生物感測晶片 |
---|---|
作 者 | 陳秀美; 林美玲; 陳慎泓; | 書刊名 | Journal of the Chinese Institute of Chemical Engineers |
卷 期 | 34:1 2003.01[民92.01] |
頁 次 | 頁151-160 |
分類號 | 460.02 |
關鍵詞 | 長鏈硫醇膜; 水膠塗覆法; 壓電生物感測晶片; Alkanethiols; Flow injection assay; Hydrocoating; Human immunoglobulin G.; Piezoelectric biochip; Protein A; Self-assembled layer; |
語 文 | 英文(English) |
中文摘要 | 本研究目的乃結合自排性長鏈硫醇膜與水膠塗覆法開發出一簡易的表面修飾技術,以提升所製備protein A壓電感測晶片於連續重複注流式檢測人類免疫球蛋白G時之穩定性與重複檢測性。首先,以尾端帶有hydrazide官能基之長鏈硫醇修飾壓電晶片之金電極表面,而後以氧化葡聚醣為架橋將protein A蛋白質固定於修飾過之電極面。利用ESCA及其他分析技術證實表面修飾的成功。此外於水膠塗覆後,晶片表面之AFM影像改變,且表面接觸角下降。檢測結果顯示,所製備之感測晶片其特異性極佳,且對人類免疫球蛋白G進行41次的連續重覆檢測後仍保有相當好之穩定性。所製備晶片在室溫乾燥環境下保存30天、在4°C緩衝液中保存14天及在檢測流槽中連續操作2天期間並作短暫保存後都仍保有活性。系統最佳之操作流速為225μl/min,且以所製備 protein A晶片對人類免疫球蛋白G進行結合動力學分析,所得之飽和頻率下降值(-△Fmax)及吸附常數(Ka)分別為297 ± 11 Hz與58 ± 19 μM¯¹。 |
英文摘要 | In order to improve the stability and reusability of protein A-coated piezoelectric biochips in a continuous repetitive flow injection assay of human immunoglobulin G (IgG), a new simple fabrication method was developed. The gold electrode surface of piezoelectric crystals was first fabricated with a self-assembled layer of hydrazide-terminated long-chain alkanethiols (15-mercpatopentadecan-l-hydrazide, 15-MH) and subsequently covalently modified with a double-sided linker of periodate-oxidized dextran, followed by protein A immobilization. The success of the surface fabrication was verified by Electron Spectroscopy for Chemical Analysis and other analyses. The AFM morphology image of the crystal surface was clearly changed and the contact angle was significantly decreased by the hydrocoating. The prepared protein A biochips exhibited remarkable specificity and fairly good stability after 41 repetitive assays in continuous flow injection assay of human lgG. The biochips remained active after 30 days of dry storage at room temperature, after 14 days in buffered solution at 4°C, and after 2 days of continuous operation with short-termed storage inside the flow detector cell. The optimal flow rate of the detection system was found to be 225 μl/min. In addition, the study of the binding kinetics of the prepared protein A biochips with human IgG revealed a -△Fmax value, the saturation frequency shift, of 297 ± 11 Hz and a Ka value, the intrinsic adsorption constant, of 58 ± 19 μM¯¹. |
本系統中英文摘要資訊取自各篇刊載內容。