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題名 | 超音波刺激與動態培養對人軟骨細胞於體外再生軟骨組織形成之影響=The Effect of Ultrasound Stimulation and Dynamic Cultivation on the Neocartilage Formation by Culturing Human Chondrocytes in Tissue-Engineering Scaffolds |
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作者 | 郭霽慶; 徐善慧; 胡淑文; 蔡清霖; | 書刊名 | 興大工程學刊 |
卷期 | 14:1 2003.03[民92.03] |
頁次 | 頁21-29 |
分類號 | 410.1644 |
關鍵詞 | 組織工程; 人軟骨細胞; 超音波; 生物反應器; Tissue engineering; Human chondrocyte; Ultrasound; Bioreactor; |
語文 | 中文(Chinese) |
中文摘要 | 本研究主要是探討超音波刺激與動態培養環境對組織工程支架中之人軟骨細胞生長成為再生軟骨的影響。首先將人軟骨細胞植入可降解型支架中,以旋轉式生物反應器與超音波刺激系統進行培養,數週後,利用生化分析方法測試支架內的細胞長個數與基質含量。結果發現支架中的細胞數量與基質含量以生物反應器培養為最佳,超音波刺激系統次之,靜置培養的狀況為最差。以組織切片染色觀察細胞與基質的量與分布,亦發現與生化分析結果一致,並且經由反應器培養後的細胞能均勻分布於支架中。以SEM觀察鍋胞型態,證實實雖然細胞在三種培養方法中皆可以維持原來的表型,但是經由反應器培養後的細胞表面有較多的基質產生。綜合以上所述,旋轉式生物反應器為最佳的體外培養系統,而超音波刺激系統亦有正面的效果。 |
英文摘要 | In this study, the effect of ultrasound stimulation and dynamic conditions on human chondrocytes growth in tissue-engineering scaffolds was examined. Chondrogenesis in 3D scaffolds was studied under control in vitro conditions. The chondrocytes harvested from adult human knee articular cartilage were seeded onto our biodegradable composite scaffolds and cultivated in vitro. After being incubated for several weeks, the chondrocytes grew well and produced more extracellular matrix in those constructs either stimulated with ultrasound or cultured in the notating-type bioreactor, especially for the latter. In addition, it was demonstrated that the chondrocytes distributed uniformly throughout the scaffolds cultured in the rotating-type bioreactor, and maintained their phenotype. In conclusion, the neocartilage could be successfully regenerated by culturing human chondrocytes in the scaffolds in the rotating-type bioreactor. Ultrasound stimulation also showed a positive effect. |
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