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題名 | 行列設計在雙染色cDNA微晶片實驗上的應用=Applications of the Row-Column Design to Two-Dye cDNA Microarray Experiments |
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作者 | 林信安; 廖振鐸; Lin, Hsin-an; Liao, Chen-tuo; |
期刊 | 中國統計學報 |
出版日期 | 20030300 |
卷期 | 41:1 2003.03[民92.03] |
頁次 | 頁1-20 |
分類號 | 368 |
語文 | chi |
關鍵詞 | 固定型模式; 基因表現; 區集設計; 實驗設計; 變異數分析; DNA; ANOVA; Block design; Experimental design; Fixed model; Gene expression; |
中文摘要 | 雙染色cDNA微晶片 (two-dyecDNA microarray) 實驗,為目前被使用來探討不同mRNA樣本之大量基因表現 (geneexpression) 差異的嶄新的生物技術。因為實驗所需的費用非 常昂貴,因此,實驗前利用統計學的實驗設計的原則,來決定好的設計更顯重要。我們考慮使用行列設計 (row-columndesign),同時來控制染劑 (dyes) 及晶片 (slides) 兩種雙染色 cDNA微晶片實驗中最主要的系統誤差 (systematic variation)。在給定的晶片數及mRNA樣本數下,依據實驗設計的原則,建構A-最適設計 (A -optimal designs) 及最佳偶設計 (best even designs)。我們將所得到的結果列表以供生物學家執行 雙染色cDNA微晶片實驗時參考使用。 |
英文摘要 | Two-dye cDNA microarray experiments now make it possible to simultaneously monitor the mRNA expression levels of thousands of genes between different biological states. As with all large-scale experiments, they can be very costly in terms of equipment, consumables and time. Therefore, how to design a good experiment, given the number of slides and the number of mRNA samples of interest, has become an important issue for this new biotechnology experiment. The variation between the two fluorescent dyes and the variation between distinct slides are thought of as two major sources of variation in a two-dye cDNA microarray experiment. In this paper, we consider the use of blocking technique, traditionally used in agricultural experiments, to control these two systematic variations. A class of A-optimal designs and the best even designs are constructed based on the fixed model of row-column design. These designs obtained should prove useful for the biologists conducting the cDNA microarray experiments. |
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