頁籤選單縮合
題 名 | The Hydrophobic-Hydrophilic Interface of Phospholipid Membranes Studied by Lamellar X-Ray Diffraction |
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作 者 | Hung,Wei-chin; Chen,Fang-yu; | 書刊名 | Chinese Journal of Physics |
卷 期 | 41:1 2003.02[民92.02] |
頁 次 | 頁85-91 |
分類號 | 330 |
關鍵詞 | |
語 文 | 英文(English) |
英文摘要 | We measured the lamellar X-ray diffraction of Dilauroyl Phosphatidylcholine (DLPE) in the form of oriented multilayers, with their water content carefully controlled by vapor pressure. Consistent diffraction patterns were recorded along the 35.0 C isotherm, with the relative humidity varying from 5% to 98%, covering both the gel phase and the fluid phase. In the gel phase, each side of the electron density profile showed a main peak and a secondary peak. The separation Ih between the two peaks was the same for both lipids and constant throughout the gel phase. These two peaks also appeared after the phase transition to the fluid phase, with the same peak separation, Ih = 4.85 Å. A comparison with crystallographic data allowed us to identify the secondary peak as the location of the hydrophobic-hydrophilic interface. Our study verifies that the molecular structure of the phospholipid headgroup region is the same in the gel phase and the fluid phase. It also provides a formula, which is practically identical to the currently accepted one proposed by other investigators, which gives the membrane's hydrophobic thickness. |
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