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題 名 | 豬眼虹膜色素上皮細胞之分離與培養=Isolation and Cultivation of Porcine Iris Pigment Epithelium |
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作 者 | 楊佩玉; 陳妙汝; 郭健龍; 陳正德; 林浤裕; 胡誕寧; | 書刊名 | 中華民國眼科醫學會雜誌 |
卷 期 | 37:2 1998.06[民87.06] |
頁 次 | 頁166-172 |
分類號 | 415.114 |
關鍵詞 | 豬眼虹膜色素上皮細胞; 細胞分離; 細胞培養; 免疫細胞化學; Iris pigment epithelium; Cell culture; Isolation; Immunocytochemistry; |
語 文 | 中文(Chinese) |
中文摘要 | 本文報告我們建立的豬眼虹膜色素上皮細胞(iris pigment epithelial cell, IPE) 細胞培養的分離與培養方法。 IPE 的分離係用酵素 -- 顯微分離法 (enzyme-microdissection method),用含胎牛血清的 F12 培養液, 在充以 5%CO �� /95% 空氣的 CO �祕菾宒楖`培養箱內培養。用本法分離培養的 IPE 存活與生長良好。 IPE 的貼 �撞v達 92%、 伸展率達 89.5%、生長率達 80%。 現已建立的七株 IPE 細胞培養已傳代 26 代,每株細胞在培養中的總分裂倍數平均為 40 次。免疫細胞化學研究顯示培養的細胞均具 有 cytokeratin 與 S-100 抗原,指示與 IPE 特性相吻合。 成功的應用本法培養豬眼 IPE 細胞, 為 IPE 體外研究提供了良好的細胞來源,並可為研究 IPE 的功能和 IPE 在各種眼 病中所起作用打下基礎。 |
英文摘要 | A method for isolation and cultivation of iris pigment epithelial (IPE) cells from porcine eyes has been developed with satisfactory results. Porcine eyes were obtained from a local slaughter house. The IPE cells were isolated by the enzyme- microdissection method, then incubated in a CO �� -regulated incubator in a humidified 5% CO �� / air atmosphere and cultured with Ham's F12 Nutrient Mixture supplemented with fetal bovine serum. These methods provide IPE cultures with good viability and rapid growth rates. The average plating efficiency, spreading rate and growth rate of isolated and cultured IPE with this method were 92%, 89.5% and 80%, respectively. Seven strains of IPE cell cultures established with this method could be passaged for an average 26 generations and for 40 population doublings. All cultured cells demonstrated cytokeratin and S-100 by immunocytochemistry, indicate that these cells are compatible with characters of IPE cells. This method provides a source for large numbers of porcine IPE cells and could be useful in studies of the biology of IPE and the role of IPE in pathogenesis of eye diseases. |
本系統中英文摘要資訊取自各篇刊載內容。