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題名 | 菊花扞插苗細菌性軟腐病之病原特性及影響病害發生之因子=Bacterial Soft Rot of Chrysanthemum Cuttings: Characteristics of the Pathogens and Factors Affecting Its Occurrence |
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作者 | 劉興隆; 徐世典; 曾國欽; | 書刊名 | 植物病理學會刊 |
卷期 | 11:3 2002.09[民91.09] |
頁次 | 頁157-164 |
分類號 | 435.435 |
關鍵詞 | 菊花扞插苗; 軟腐細菌; 細菌性軟腐病; Chrysanthemum cutting; Erwinia chrysanthemi; Erwinia carotovora subsp; Carotovra; Bacterial soft rot; |
語文 | 中文(Chinese) |
中文摘要 | 自彰化縣田尾鄉菊花扞插苗細菌性軟腐病罹病組織分離到32個Erwinia軟腐細菌菌株,經生理生化特性測定結果,其中有23個菌株為E. carotovora subsp. Carotovora,其餘9個菌株為E. chrysanthemi,進一步鑑定E. chrysanthemi菌株,顯示其中有8個菌株屬於subdivisionII及biovar 6;另一個菌株則屬於subdivision IV及biovar 3。溫度可影響菊花扞插苗細菌性軟腐病之發生,在25-30℃下Erwinia軟腐細菌引起之扞插苗軟腐長度明顯較15-20℃時為長,其腐爛倒伏之情形亦較嚴重;此外栽培介質含水量愈高造成菊花扦插苗之軟腐愈嚴重;而菊花插穗癒合時間愈長其軟腐病之發生則較輕微。以modified crystal violet pectate (CVP)選擇性培養基測菊花母株之灌溉水、土壤及菊花頂芽Erwinia軟腐細菌之存在情形,結果顯示Erwinia軟腐細菌於此些樣品中出現之頻率分別為100%、15%及7%。 |
英文摘要 | A total of 32 strains of soft rot Erwinia were isolated from rooted tissues of chrysanthemum cuttings in Tienwei, Changhua. Based on the results of physiological and biochemical tests, 23 strains were identified as Erwinia carotovora subsp. Carotovora, and 9 strains as Erwinia chrysanthemi. The strains of E. chrysanthemi were further characterized according to Dickey's and Boccara's systems, eight strains were classified to the subdibision II and biovar 6, while one strain belonged to the subdibision IV and biovar 3. Temperature could affect the soft rot severity of chrysanthemum cuttings caused by soft rot Erwinia. Length of pith maceration of cuttings was significantly longer at 25-30℃ than that at 15-20℃. Water content of culture medium was also found to affect soft rot development. Soft rot of chrysanthemum cuttings was more severe when the cuttings were planted in culture medium with higher water content. Severity of soft rot of chrysanthemum cuttings was found to be reduced if the cuttings were kept at high moist conditions for curing before planting. A modified CVP selective medium was used to study the presence of soft rot Erwinia in mother stock fields of chrysanthemum. Soft rot Erwinia could be detected from irrigation water, soil and chrysanthemum apical shoot with frequency at 100%, 15% and 7%, respectively. |
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