查詢結果分析
來源資料
相關文獻
- 莧菜葉枯病菌之鑑定與侵染過程
- 多用途蔬菜清洗機之試驗改良
- 臺灣欒樹圓尾蚜(Periphyllus koelreuteriae (Takahashi))之完全生活環及各蟲期之形態特徵
- Notes on the Flora of Taiwan(10): Acalypha Brachystachya Hornem. and its Related Species (Euphorbiaceae)
- 溫度對欒樹圓尾蚜(Periphyllus koelreuteriae)(同翅目:毛蚜科)有性世代各型蚜發育、壽命及繁殖之影響
- 莧菜品種改良
- 本省莧菜栽培簡介
- 不織布在蕹菜及莧菜冬季防寒栽培之研究
- 蘭陽地區夏季短期葉菜類種子預措、播種密度及覆蓋材料之研究
- 莧菜白銹病之感染源與其病勢之進展
頁籤選單縮合
題名 | 莧菜葉枯病菌之鑑定與侵染過程=Characterization and Infection of Thanatephorus Cucumeris Causing the Chinese Amaranth Foliage Blight |
---|---|
作者 | 林信甫; 謝廷芳; 黃振文; | 書刊名 | 植物病理學會刊 |
卷期 | 11:1 2002.03[民91.03] |
頁次 | 頁33-44 |
分類號 | 435.24 |
關鍵詞 | 莧菜; 莧菜葉枯病; 立枯絲核菌AG 2-2 IIIB; 有性世代; 擔孢子; 侵染過程; Anastomosis basidiospore; Chinese amaranth; Foliage blight; Infection process; Rhizoctonia solani AG 2-2 IIIB; Thanatephorus cucumeris; |
語文 | 中文(Chinese) |
中文摘要 | 臺灣地區有機農場生產的莧菜(Amaranthus mangostanus L.)栽培田,最近出現莧菜葉枯病(Rhizoctonia foliage blight),按照柯霍氏法則(Koch's postulates)系列測試,證實它的病原菌是Rhizoctonia solani Kuhn [有性世代Thanatephorus cucumeris (Frank) Donk]。將莧菜葉枯病菌R. solani RSA-03和RSA-09與R. solani標準菌株行菌絲融合群測定,發現兩菌株與R. solani AG 2-2 IIIB具有高菌絲融合率(>70%);與標準菌株R. solani AG 2-1、AG 2-2IV、AG2-3及AG BI的菌絲融合率則小於5%。探討兩菌株對於硫胺素(thiamine-HCI)的需求,發現莧菜葉枯病菌與標準菌株R. solani AG 2-2 IIB皆屬於營養缺陷菌株。另外莧菜葉枯病菌與標準菌株R. solani AG2-2 IIIB的菌絲生長溫度及生長速度相近,且此標準菌株的菌絲塊對莧菜亦具有病原性,因此將莧菜葉枯病菌鑑定為R. solani AG 2-2 IIIB。土壤覆蓋法能誘使莧菜葉枯病菌RSA-03和RSA-09大量產生擔孢子。以擔孢子懸浮液接種於株齡四星期的莧菜植株,在28℃的濕室中保濕,六天後葉片開始出現水浸狀病徵。受害莧菜葉片初期病斑呈圓形,水浸狀的透化小斑,直徑大小約1mm左右,接著病斑擴展為後期不規則的爪狀斑;病勢擴展迅速時,病斑間會相互癒合,造成葉片枯萎死亡。利用光學與螢光顯微鏡,觀察擔孢子侵染莧菜葉片的過程,發現接種擔孢子9hr後,發芽管會侵入葉片,18hr後團狀菌絲形成,直到第21hr團狀菌絲體會逐漸形成子座般的構造(stroma-like structure),並存在於病斑中央。 |
英文摘要 | A new foliage blight of Chinese amaranth (Amaranth mangotanus L.) was frequently observed in organic farms during the summer season in Taiwan. The hyphae of RSA-03 and RSA-09 isolated from diseased Chinese amaranth leaves were able to form anastomosis in high frequency (>70%) with R. solani AG 2-2 IIIB, but in low frequency (<5%) with R. solani AG 2-1, AG2-2 IV, AG2-3 and AG BI. When five isolates from Chinese amaranth were individually cultured in liquid glucose asparagines (GA) medium with or without thiamine-HCI, all of them were found to be auxotrophic for thiamine-HC1 and more closely resembled R. solani AG 2-2 IIIB. The optimum temperature for mycelial growth of RSA-09 was also similar to that of R. solani Ag 2-2 IIIB. Inoculation tests revealed that RSA-03, RSA-09 and R. solani AG 2-2 IIIB were pathogenic to Chinese amaranth. Based on the anastomosis, thiamine-HC1 requirement, growth temperature, and pathogenicity tests, the isolates from Chinese amaranth foliage blight were identified as R. solani AG 2-2 IIIB. When Chinese amaranth plants were sprayed with basidiospores produced by RSA-03 or RSA-09, small, circular water-soaked spots appeared on leaves within six days. Subsequently, the lesions enlarged and became necrotic and irregular in shape. Basidiospores germinated on leaves and penetrated into the epidermal cell walls nine hours after inoculation. The pathogen formed mycelial masses in 18 hr and stroma-like structures in 21 hr after inoculation. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。