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題 名 | Compound 48/80-induced Degranulation of GnRH-like Immunoreactive Mast Cells in the Brain and Mesentery of the Gerbil=化合物48/80誘發的沙鼠腦及腸繫膜內具GnRH免疫反應之肥大細胞的去顆粒化 |
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作 者 | 楊美芳; 錢宗良; 盧國賢; | 書刊名 | 動物研究學刊 |
卷 期 | 41:1 2002.01[民91.01] |
頁 次 | 頁99-110 |
分類號 | 364 |
關鍵詞 | 去顆粒化; 肥大細胞; 促性腺激素釋放激素; 腦; 腸繫膜; Degranulation; Mast cell; GnRH; Brain; Mesentery; |
語 文 | 英文(English) |
中文摘要 | 肥大細胞可經由去顆粒化(degranulation)而影響其胞外環境。近來的研究結果顯示,鴿子及哺乳類的肥大細胞可表現促性腺激素釋放激素(Gonadotropin-relasing homone, GnRH)的免疫反應。本研究的目的在探討GnRH於腦及腸繫膜肥大細胞的表現,以及投予化合物40/80後其顆粒化情形本研究採用沙鼠為實驗動物,因沙鼠的腦及腸繫膜內具有大量肥大細胞。本研究之結果顯示:腦及腸系膜的肥大細胞,不論是對照組或投藥組皆可表現GnRH的免疫反應。腸繫膜肥大細胞於腹腔內投藥後有非常劇烈的去顆糖化反應。電子顯微鏡觀結果顯示,沙鼠之腦及腸繫膜肥大細胞所釋放具有GnRH免度反應的顆粒都相當緻密,且釋放顆粒後的肥大細胞可於投藥後十二小時復原。由上述結果可得以下結論:(一)腦及腸繫膜肥大細胞皆可表現GnRH 的免疫反應,以及(二) GnRH是以快速的釋放反應經由肥大細胞釋放出來。 |
英文摘要 | Mast cells affect the extracellular milieu through the process of degranulation. Recently, mast cells in the brain of the dove and certain mammals were found to express gonadotropin-relasing homone (GnRH)-like immunoreactivity (IR). In the present study, we examine the expression of GnRH-like IR in brain and mesenteric mast cells, and the degraulation of mast cells at these 2 sites after administration of compound 40/80 (C40/80). The gerbil, in which numberous mast cells are found in the brain and mesentery, was used as the experimental animal model. Both brain and mesenteric mast cells in vontrol and C48/80-treated gerbils express GnRH-like IR. Mast cell degranulation was dramatic in the mesentery after C48/80 was injected intraperitoneally. Because newly extruded GnRH-like immunoreactive granules in the brain and mesentery are compact, GnRH release can be classed as a rapid0-release reaction. The degranulated mast cells were restored to their normal configuration in the brain and mesentery 12 h after C48/80 treatment. We conclude that: (1) brain and mesenteric mast cells express GnRH –like IR, and (2) GnRH is released by a rapid-release reaction after C48/80 administration. |
本系統中英文摘要資訊取自各篇刊載內容。