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題名 | LH-Immunoreactivity and Ultrastructural Changes in the Pituitary Gland of Post-Castrated Rats: Quantitative and Morphologic Studies=大白鼠去勢後腦下重體之黃體促素免疫染色反應與超微結構變化:形態計量與形態學之研究 |
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作者 | 游祥明; 游松霖; 邱美妙; 林光華; Yu, Shang-ming; Yu, Sung-ling; Chiu, Mei-miao; Lin, Kwan-hwa; |
期刊 | 動物研究學刊 |
出版日期 | 19970700 |
卷期 | 36:3 1997.07[民86.07] |
頁次 | 頁230-239 |
分類號 | 414.82 |
語文 | eng |
關鍵詞 | 去勢; 免疫細胞化學; 黃體促素; 腦下垂體; 形態計量; Castration; Immunocytochemistry; LH; Luteinizing hormone; Pituitary; Morphometry; |
中文摘要 | 在去勢後第一天,雌性大白鼠的腦下垂體的黃體促素免疫染色反應細胞並不膨大 且對照組相同,而在雄性大白鼠的腦下垂體卻具有一些膨大的黃體促素免疫染色反應細胞, 即具有一些更加膨脹的粗糙內質網小池。在去勢後第二天,在雌性大白鼠的腦下垂體內可發 現一些膨大的黃體促素免疫染色反應細胞。此時在雄性大白鼠的腦下垂體內膨大的黃體促素 免疫染色反應細胞含有空泡存在,亦即含有一些更加膨脹的粗糙內質網小池。在去勢後第四 天,在雌性大白鼠的腦下垂體內膨大的黃體促素免疫染色反應細胞也含有空泡存在。但在雄 性大白鼠的腦下垂體內膨大的黃體促素免疫染色反應細胞含有融合性空泡,亦即含有一些更 加膨脹融合的粗糙內質網小池。在去勢後第七天,在雌性大白鼠的腦下垂體內有許多膨大的 黃體促素免疫染色反應細胞也含有空泡存在。但在雄性大白鼠的腦下垂體內許多膨大的黃體 促素免疫染色反應細胞卻含有融合性空泡,亦即含有一些更加膨脹更顯著融合的粗糙內質網 小池。在去勢後第十四天,在雌性大白鼠的腦下垂體內有更多膨大的黃體促素免疫染色反應 細胞也含有空泡。但在雄性大白鼠的腦下垂體內有更多膨大的黃體促素免疫染色反應細胞相 互排列並連接成不規則的索狀構造,並且一些膨大的黃體促素免疫染色反應細胞含有融合性 空泡。在雌性和雄性大白鼠兩者的腦下垂體內,大部份的膨大的性腺細胞含有較小的分泌顆 粒,僅有一小部份的膨大的性腺細胞含有較大的分泌顆粒。 |
英文摘要 | Shang-Ming Yu, Sung-Ling Yu, Mer-Miao Chiu and Kwan-Hwa Lin (1997) LH- Immunnoreactivity and ultrastructural changes in the pituitary gland of post-castrated rats: quantitative and morphologic studies zoological Studied 36(3): 230-239. At 1-d postcastration of 2- mo-old rats, LH-immunoreactive (IR) cells were not hypertrophied in the female pituitary gland but a few LH-IR cells were hypertrophied in that of males. At 2-d postcastration, a few LH-IR cells were hypertrophied in the female pituitary gland and contained small cytoplasmic vacuoles in males. At 4- d postcastration, a few hypertrophied LH-IR cells contained a few small cytoplasmic vacuoles in the female pituitary gland and contained coalesced vacuoles in males. At 7-d postcastration, numerous hypertrophied LH-IR cells contained a few small cytoplasmic vacuoles in the female pituitary gland and contained coalesced vacuoles in males. At 14-d postacstration, a large number of hypertrophied LH-IR cells contained numerous cytoplasmic vacuoles in the female pituitary gland and had become arranged in irregular, interconnecting cords in males. At the electron microscopic level, cisternae of the rough endoplasmic reticulum (RER) were slightly dilated in non-hypertrophied gonadotrophs of the female pituitary gland but moderately dilated in hypertrophied gonadotrophs of males at 1-d postcastratin. At 2-d postcastration, cisternae of the RER were slightly dilated in hypertrophied gonadotrophs of the female pituitary gland and moderately dilated in those of males. At 4-d postcastration, cisternae of the RER were moderately dilated in hypertrophied gonadotrophs of the female pituitary gland and had coalesced in those of males. At 7-d postcastration, cisternae of the RER remained moderately dilated in hypertrophied gonadotrophs of the female pituitary gland and had coalesced and markedly dilated in those of males. At 14-d postcastration, cisternae of the RER were similar to those in hypertrophied gonadotrophs of female and male pituitary glands at 7-d postcastration. Most of the secretory granules were small in size but a few large ones were also present in the cytoplasm of female and male hypertrophied gonadotrophs. |
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