頁籤選單縮合
題 名 | 臺灣鐵杉之組織培養=Tissue Culture of Tsuga Chinensis var. formosana |
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作 者 | 成寧; 王亞男; | 書刊名 | 國立臺灣大學農學院實驗林研究報告 |
卷 期 | 15:4=234 2001.12[民90.12] |
頁 次 | 頁303-315 |
分類號 | 436.11 |
關鍵詞 | 臺灣鐵杉; 成熟胚; 癒合組織; 不定芽; 小植株; Tsuga chinensis; Mature embryo; Callus; Adventitious bud; Plantlet; |
語 文 | 中文(Chinese) |
中文摘要 | 本試驗以臺灣鐵杉(Tsuga chinensis (Franchet) Pritz. Ex Diels var. formosana (Hayata) Li & Keng)之成熟胚為培植體進行培養。將成熟種子先以流水處理48小時,再以70%酒精浸泡兩分鍾,最後在5%NaOCI水溶液(含約1% (v/v) Tween20展著劑)中超音波震盪20分鐘,可達零污染率。取成熟胚接種於含0.1%(v/v)EM-X溶液之培養基培養,可大幅提高其發芽率及正常生長。以MS培養基在光照環境下,添加0.1ppm TDZ培養可促進幼苗生長。以MS為基礎培養基在黑暗的環境下,添加1ppm BA與2ppm 2,4-D培養,可獲得大量淡褐色、鬆軟之癒合組織。以SH培養基培養,添加BA、TDZ或BA與2,4-D的組合,皆可誘導不芽再生。在黑暗的環境下,BA與2,4-D則可誘導出白色堅硬的癒合組織。將具不定芽之培植體移往不含生長調節劑1/2SH培養基,以含活性炭與不含活性炭的培養基交互培養,可得小植株。 |
英文摘要 | Mature seeds of Tsuga chinensis var. formosana were treated with running water for 48 hours ad then soaked in 70% ethanol solution for 2 minutes. The seeds with 5% NaOCI (supplemented with 1%(v/v) Tween 20) were kept in an ultrasonic shaker for 20 minutes, and the result was zero contamination. The culture medium containing 0.1% (v/v) EM-X promoted germination rate of excised embryos. The seedlings grew better on MS medium containing 0.1ppm TDZ under light condition. Light brownish soft calli were induced after mature embryos were cultured on MS medium containing 1ppm BA and 2ppm 2,4-D under dark condition. Adventitious buds formed on SH medium containing BA or TDZ with 2,4-D. Under dark condition, white compact calli were induced in SH medium and treated alternatively with and without 0.1% (w/v) activated charcoal, the adventitious buds grew into plantlets. |
本系統中英文摘要資訊取自各篇刊載內容。