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題名 | The Effect of Combined 5-Fluorouracil and Dexamethasone on Cultured Human Retinal Pigment Epithelial Cells=合併使用抗增殖性藥物與類固醇對於培養之人類視網膜色素細胞之抑制效果 |
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作者 | 童逸楨; 吳文權; 高英賢; 張祐誠; 鍾佳宏; 胡誕寧; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
卷期 | 17:10 2001.10[民90.10] |
頁次 | 頁524-529 |
分類號 | 416.746 |
關鍵詞 | 抗增殖性藥物; 類固醇; 人類視網膜色素細胞; Proliferative vitreoretinopathy; Human retinal pigment epithelium; 5-fluorouracil; Dexamethasone; |
語文 | 英文(English) |
中文摘要 | 本研究乃是針對培養之人類視網膜色素細胞(human retinal pigment epithelial cell)評估合併使用抗增殖性藥物(5-fluorouracil,5-FU)與類固醇 (dexamethasone, DEX)對其所產生的抑制增殖效果。 人類視網膜色素細胞被培 養之後, 加入各種不同濃度的5-FU(0,250,500,1000,2000 ng/ml)與DEX (0,1,10,100,200 μg/ml) , 總共培養96小時,於第48小時更換其培養基以維持 藥物的效價。我們使用 trypan blue exclusion method 計算存活細胞的數目,並利 用活細胞的mitochondrial dehydrogenase能將tetrazolium salt 代謝成formazan dye 的特性來評估細胞的增殖。我們發現5-FU單獨使用時其抑制效果與劑量成正 比,並得到5-FU的 一半致死劑量(IC50)為704.12ng/ml,而DEX單獨使用時, 對於人類視網膜色素細胞具有雙重效果-低濃度(1,10μg/ml)能刺激而高濃度 (100,200μg/ml)能抑制其增殖,當兩者合併使用時並沒有比分別單獨使用來的有 效,除非DEX的濃度大於200 μg/ml才有加成抑制的效果。 |
英文摘要 | This study was designed to investigate the inhibitory effect of 5-fluorouracil (5-FU) and dexamethasone (DEX) on the proliferation of human retinal pigment epithelial ( RPE ) cells in vitro. The human RPE cells ( R-50 cell line ) were cultured and exposed to various concentrations of combined 5-FU (0, 250, 500, 1000, 2000 ng/ml) and DEX (0,1,10,100,200 μg/ml). The cells were incubated for 96 hr and the medium was changed every 48 hr to replenish the drug action. Cell viability was assessed using cell counting and trypan blue exclusion method. Tetrazolium salt, which can be metabolized by mitochondrial dehydrogenase to form a formazan dye, was used to assay cell proliferation. Treatment with 5-FU alone inhibited cell proliferation in a dose-dependent manner. The concentration of 5-FU that inhibited growth by 50% (IC50) was found to be 704.12 ng/ml. There was a bimodal effect of DEX on RPE cells-stimulation at low concentrations (1,10 μg/ml) and inhibition at high concentrations (100,200μg/ml). When the two drugs were combined, there was additive inhibition in the concentration of 200 μg/ml of DEX. These results indicate that a combination of 5-FU and DEX is no more effective in the inhibition of human RPE cells, except in combination with high concentrations of DEX (≧200μg/ml). |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。