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題 名 | 高壓氧治療對缺血-再灌流傷害之效應:大白鼠島狀皮瓣模式=Effects of Hyperbaric Oxygen Therapy on Ischemic-reperfusion Injury: A Rat Island Flaps Model |
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作 者 | 林燈賦; 陳清本; 牛柯琪; 卓妙如; 王先震; 李信慧; | 書刊名 | 醫學研究 |
卷 期 | 21:專刊 民90.11 |
頁 次 | 頁87-97 |
分類號 | 418.992 |
關鍵詞 | 大白鼠腹部島狀皮瓣; 皮瓣存活率; 高壓氧治療; 缺血-再灌流傷害; 中性球; Epigastric island flaps; Flap survival rate; Hyperbaric oxygen therapy; Ischemia-reperfusion injury; Neutrophils; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究利用大白鼠腹部島狀皮瓣,建立缺血-再灌流的組織傷害模型,來探討高壓氧治療對缺血-再灌流傷害的效應。將體重250-350克的雄性SD大白鼠隨機分組為六組;第一組:對照組;第二組:對照組+HBO₂;第三組:缺血再灌流組;第四組:缺血再灌流組+灌流後HBO₂;第五組:缺血再灌流組+灌流前HBO₂;第六組:缺血再灌流組+灌流前HBO₂+灌流後HBO₂。大白鼠在麻醉狀況下接受手術,在左腹壁製造4×5 cm²大小的島狀皮瓣,皮瓣與其下方之皮下組織以一生物膜(Biobrane)相隔,以阻斷皮瓣之營養供給。對照組大白鼠有分離但未經缺血-再灌流傷害之皮瓣,且置於高壓艙接受模擬高壓曝露但無高壓氧治療。缺血再灌流組大白鼠以血管夾夾住其下腹上血管十小時以阻隔皮瓣之血流供應,作為缺血-再灌流機制。高壓氧治療(3ATA, 100% O₂)每次60分鐘,每天依各組實驗動物所需給予,連續五天。灌流前高壓氧治療於組織缺血三小時後尚未再灌流前給予,灌流後高壓氧治療則於組織缺血十小時後再灌流時馬上給予。在皮瓣手術後第七天,取其皮瓣組織並計數其存活率及皮瓣內中性球的數目。 結果顯示皮瓣組織存活率及皮瓣內中性球的數目於缺血再灌流組分別為1.09±0.62%,367.63±19.06 (mean±SEM);對照組為99.94±0.03%,93.38±6.44;對照組+高壓氧治療為100±0.00%,117.38±2.63;缺血再灌流組+灌流後HBO₂為53.83±3.37%,208.25±17.89;缺血再灌流組+灌流前HBO₂為27.98±3.52%,177.13±18.38;缺血再灌流組+灌流前HBO₂+灌流後HBO₂為68.21±3.47%,139.88±7.95。缺血再灌流傷害有顯著地增加皮瓣內中性球的數目及減少皮瓣組織存活率(p<0.01)。再灌流前高壓氧治療與灌流後高壓氧治療均能顯著地減少皮瓣內中性球的數目及增加皮瓣組織存活率(p<0.01)。研究結果指出再灌流前之高壓氧治療與灌流後之高壓氧治療均對減少缺血再灌流傷害,增加大白鼠島狀皮瓣組織的存活率有顯著效應,且兩者有加成性的作用。 |
英文摘要 | This study was to investigate the effects of hyperbaric oxygen (HBO₂) therapy to the ischemia-reperfusion (I/R) injury through epigastric island flaps of rats. Male Sprague-Dawley (SD) rats weighing between 250-350g were randomly assigned into one of six groups (n=8 in each group): Control (G1), Control + HBO₂ (G2), I/R (G3), I/R + HBO₂ (G4), Early HBO₂ treatment + I/R (G5), Early HBO₂ treatment + I/R + HBO₂ (G6). All rats were subjected to surgery of cutting left abdominal wall with 4.0 × 5.0 cm² in area for island flap with only preserved inferior epigastric vessels under anesthesia. Biobrane membranes were placed between flaps and underlined recipient beds. Ischemia-reperfusion injury was induced by occlusion with vessel clips on the inferior epigastric vessels for 10 hours. Early HBO₂ treatment was performed after 3-hr ischemia before flap reperfusion. HBO₂ after I/R was performed once immediately after reperfusion and once a day for 5 successive days. Neutrophils in flap tissues were counted under microscopy, and the overall survival rate of island flaps was measured at the seventh day after operation. The survival rate of flaps and leukocyte counts subjected to I/R (G3) were 1.09 ± 0.62 %, 367.63 ± 19.06 (mean ± SEM); 99.94 ± 0.03 %, 93.38 ± 6.44 for G1; 100 ± 0.00 % , 117.38 ± 2.63 for G2; 53.83 ± 3.37 %, 208.25 ± 17.89 for G4; 27.98 ± 3.52%, 177.13 ± 18.38 for G5; and 68.21 ± 3.47 %, 139.88 ± 7.95 for G6, respectively. Ischemia-reperfusion injury has significantly increased leukocyte counts locally in flap tissues and decreased the overall survival rate of flaps compared with control (p < 0.01, ANOVA). Early HBO₂ treatment and HBO₂ after I/R have significantly decreased leukocyte counts in flap tissues and increased the overall survival of I/R flaps (p < 0.01, ANOVA). These results indicated that early HBO₂ treatment and HBO₂ after I/R can additively improve island flap survival rate from I/R injury in rats. |
本系統中英文摘要資訊取自各篇刊載內容。