查詢結果分析
相關文獻
- Protective Effects of Partially Purified Antigens of Actinobacillus Pleuropneumoniae on Experimentally Infected Mice
- 核酸混合物對小白鼠學習記憶能力之影響
- 不同的血清或培養液對小白鼠胚胎生體外發育的比較
- Effect of Dietary Protein Level on Passive Avoidance Performance and Brain Amino Acids in Senescence-Accelerated Mice
- ATP Sensitive Potassium Channel and Myocardial Preconditioning
- Toxicity Assay of Cyanobacterial Strains Using Artemia Salina in Comparison with the Mouse Bioassay
- 豬博德氏菌、巴氏桿菌、放線桿菌、丹毒絲狀菌及假性狂犬病不活化五合一混合疫苗之研製與田間應用試驗
- Edwardsiella tarda對於試驗動物之致病性與試製菌苗以小白鼠效力測定模式之研究
- Effects of Cadmium Nitrate on Locomotor Behaviors and Organs in Male Adult Mice
- 核苷與核苷酸混合物對老化促進小白鼠學習記憶能力之影響
頁籤選單縮合
題 名 | Protective Effects of Partially Purified Antigens of Actinobacillus Pleuropneumoniae on Experimentally Infected Mice=部份精製的胸膜肺炎放線桿菌(Actinobacillus pleuropneumoniae)抗原對實驗染小白鼠的保護作用 |
---|---|
作 者 | 劉建榮; 鄭益謙; 陳啟銘; 翁仲男; | 書刊名 | 微免與感染雜誌 |
卷 期 | 32:4 1999.12[民88.12] |
頁 次 | 頁223-232 |
分類號 | 437.245 |
關鍵詞 | 胸膜肺炎放線桿菌; 小白鼠; 保護作用; Actinobacillus pleuropneumoniae type 1; Partial purification and characterization; Protective effect on mouse; |
語 文 | 英文(English) |
中文摘要 | 以含有0.5ug/mL的Nacotiamide Adenosine Diphosphate(NAD)腦心浸出培養液(Brain Heart Infusion broth-NAD,BHI-NAD)18小時培養的胸膜肺炎放線桿菌(AP)血清型1型之上清液及其離心沉澱的菌塊做部份地精製,然後以三種模型做對實驗感染小鼠(Balb/c)的保護試驗。AP培養液上清液則以Polyethylene glycol 6000沉澱法(此抗原叫CCSE),而離心沉澱的菌塊則以溶菌後,通過Sephade G-200之方法(此抗原叫PPCE),做部份地精製。CCSE與PPCE中所含的蛋白質與五碳醣量約各為0.2-0.17及0.014-0.012 mg/ml。SDS-PAGE分析時,CCSE出現至少有10條較清楚的蛋白質帶,其分子量各約為110,104,68,50,45,40,35,30,24及20 Kilodaltons(KDa),而PPCE則分子量比40KDa大的區域,則模糊而缺少110KDa的帶(此帶為代表菌莢膜的主要成份)。單以CCSE或PPCE免疫得到的抗體價,比以整菌(whole cell)或SE混合CE免疫所得到的凝集或ELISA抗體價為低。培養18小時的上清液中的溶血價(以豬血球測定)很低,而經精製變CCSE後容血作用則完全消失。皮下免疫對10LD�t菌量(約6x10�顏FU/50 uL)鼻內攻擊之模型下,只有整菌及SE混合CE免疫群有微弱的保護作用,鼻內免疫對以20LD�t菌量(約1.2x10�eCFU/50 uL)鼻內攻擊之模型下,則只有以1LD�t以上菌量免疫群有微弱的保護作用。肌肉免疫對以2或6LD�t(約1.2x10�瘠FU/0.5 mL或3.6x10�瘠FU/0.5mL)菌量腹腔打針攻擊之模型下,上述的四種抗原均顯出有相當高的保護作用。 |
英文摘要 | Three different models of protection experiments in mice using partially purified Actinobacillus pleuropneumoniae antigens such as crude culture supernatant extract (CCSE) and partially purified cell extract (PPCE) were attempted. Biochemical analysis showed that these two immunogens had protein concentration of 0.17-0.2 mg/mL and pentose concentration of 0.012-0.014 mg/mL. In the first model intranasal (IN) vaccination with different doses (from 0.01-10 IN-LD�t) against In challenge with the dose of 20 IN-LD�t containing 1.2x10�� colony forming unit (CFU)/50uL showed that only those with the dose more than 1 IN-LD�t had slight protection in terms of survival index (SI). In the second model of protection experiment, in which subcutaneous vaccination (SC) with the immunogens plus soybean oil against IN challenge with 10 IN-LD �t containing 6x10CFU/50uL, showed that formalin-killed bacteria (bacterin) and CCSE plus PPCE had only a slight protection whereas vaccination with CCSE or PPCE immunogen alone had no protection. In the third model of protection experiment, in which the intramuscular (IM) vaccination with the immunogens plus aluminum hydroxide [Al (OH��)]gel against intraperitoneal (IP) challenge with the dose of either 2 or 6 IP-LD�t containing 1.2-3.6x10�� CFU/0.5 mL of 0.3% mucin saline showed highly effective. |
本系統中英文摘要資訊取自各篇刊載內容。