頁籤選單縮合
題名 | Construction of a Recording-Injection Microelectrode with Microwire=利用微金屬絲製造可同時給藥與記錄之微電極 |
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作者 | 蕭富仁; 嚴震東; 陳瑞芬; | 書刊名 | Acta Zoologica Taiwanica |
卷期 | 12:1 民90.01 |
頁次 | 頁21-29 |
分類號 | 437.23 |
關鍵詞 | 微金屬絲; 單一神經元記錄; 微量注射; Microwire; Single-unit recording; Stereode; Microinjection; |
語文 | 英文(English) |
中文摘要 | 本研究提供一簡單的方法,利用市售的玻璃管與絕緣的鎳微金屬絲,製造可同時局部微量給藥並記錄神經動作電位之微電極。同時本研究也提出一種不需要藉由任何特別助焊劑輔助的微金屬絲焊接方式。此種可同時局部微量給藥與記錄之微電極可以穩定地記錄到不同腦核的單一神經細胞電位活性,而且此單一神經電位活性會隨著微量給予的麩胺酸(glutamate, 10 mM)量的增加而反應增強。另外,本研究之微電極製作方式亦可應用於雙記錄電極(stereode)之製作。本研究並顯示雙電極記錄方式可以成功地降低雜訊,以提昇單一神經細胞活性記錄之訊號品質。本論文所提供之微電極製作方式,相信也可以成功地應用於多管微量注射並同時記錄電位活性之微電極製作。 |
英文摘要 | A simple method for the construction of a recording-injection microelectrode (RIM) is described here. We used commercially available materials, including formvar-insulated nichrome microwire and a glass pipette. We also used a method for microwire soldering without the assistance of any specific solder-aid material to construct the RIMs. The RIM performed consistently at different depths, and yielded stable recordings of single units in the medulla of the rat. A glutamate solution (Glu) at 10 mM was microinjected. An increase in neural activity was consistently obtained as the injected volume of Glu increased. Therefore, this microelectrode assembly was proven to be useful in recording single-unit activity of the brain. A stereode configuration is also described in the present study. Significant noise reduction was achieved with this configuration. Our RIM can easily be combined with a glass multi-barrel electrode to form a multiple recording-injection microelectrode unit. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。