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題名 | Genetic Identification of Chinese Drug Materials in Yams (Dioscorea spp.) by RAPD Analysis=以RAPD法鑑定山藥藥材之基原 |
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作者 | 賴宏亮; 劉宏仁; 廖明輝; 陳建志; 劉新裕; 許博文; | 書刊名 | 藥物食品分析 |
卷期 | 9:3 2001.09[民90.09] |
頁次 | 頁132-138 |
分類號 | 414.32 |
關鍵詞 | 植物源; 山藥; 基因親源關係分析; Botanical origins; Yam; Dioscorea spp.; Phylogenetic analysis; |
語文 | 英文(English) |
中文摘要 | 以RAPD法檢測薯蕷屬(Dioscorea)18個品系山藥藥材的基原。以30個隨機引子進行 聚合�t連鎖反應增幅山藥藥材葉子之 DNA。30 個引子中的 18 個引子可產生 237 個基因標 誌。 其中的四個引子 OPP-40,OPP-15, OPAP-04 及 OPAP-10 可用於區分這 18 個品系之 山藥藥材。計算品系間之相似與相異係數及分群分析可建構品系間之基因親綠關係圖,並可 將 18 個品系分為 5 個次群。 基因相似及相異度分析顯示 70W37 品系與 70W47 品系差異 較大,而 70W41 品系則與 70W59 品系較為相似。本研究已成功的應用 RAPD 法鑑定及區別 薯蕷屬的 18 個品系山藥藥材。 |
英文摘要 | The botanical origins of Chinese drug materials in yams were examined among eighteen cultivarities/species of Dioscorea by randomly amplified polymorphic DNA (RAPD). DNA isolated from fresh leaves were used as a template in polymerase chain reactions (PCR) using thiry random decamer primers. Eighteen primers produced 237 polymorphic RAPD markers. which were scored for all the lines studied. Four primers including OPP-04, OPP-15, OPAP-04 and OPAP-10, could distinguish all tested samples. Genetic distances and similarities among eighteen cultivarities/species were calculated, and a cluster analysis was used to generate a dendrogram showing a phylogenetic relationship. The studied Dioscorea species could be divided into five subgroups by phylogenetic analysis. Genetic distance and similarity analysis showed that line 70W37 was distantly related to 70W47, and line 70W41 was closely related to 70W59. RAPD technique could be successfully applied for the rapid identification and differentiation of Dioscorea species. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。