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題 名 | Antimutagenicity of Extracts of Hericium Erinaceus=猴頭菇萃取物抗突變性之研究 |
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作 者 | 王進琦; 胡淑惠; 李維綸; 蔡麗玉; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
卷 期 | 17:5 2001.05[民90.05] |
頁 次 | 頁230-238 |
分類號 | 341.91 |
關鍵詞 | 猴頭菇; 萃取物; 抗突變性; Antimutagenicity; Mutagenicity; Hericium erinaceus; |
語 文 | 英文(English) |
中文摘要 | 猴頭菇在食用及藥用方面均有實際的價值,內含的水溶性多醣類,可 促進免疫及抗人工轉移肺腫瘤之效果。本研究以液體培養菌絲及固體培養子實體 之水及乙醇萃取物,檢測其抗突變效果;使用五種的致突變劑,AFB1、B[a]P、 Glu-P-1、NQNO及Trp-P-1,再利用Ames test方法,以Salmonella typhimurium TA98為試驗菌,探討四種萃取物之抗突變作用。結果發現,對Trp-P-1的抗致 突變力最好,其次為Glu-P-1、B[a]P、AFB1,而對NQNO的效果最差。隨濃度之 增加,它們的抗突變力會漸增;當濃度達到200ppm時,作用呈現最高。可是, 各濃度間卻無線性關係 (p>0.05)。若將萃取物加熱處理,其抗突變作用就會明顯 的下降(p<0.05),此顯示這些萃取物之抗突變效果為熱不安定性。菌絲及子實 體之乙醇萃取物對所有致突變劑之抗突變作用皆優於水萃取物者(p<0.05)。而 且,子實體的抗突變效果,不論是水或乙醇萃取物,均比菌絲者要好。 |
英文摘要 | Hericium erinaceus is valuable in the diet and in medical treatment. It contains water-soluble polysaccharides that have been found to enhance immunity and which show anti-artificial pulmonary metastatic tumor effects. In this study, water and ethanol extracts of the mycelium and fruiting body of Hericium erinaceus were examined by the Ames test using Salmonella typhimurium TA98 to screen for antimutagenic effects against 5 mutagens: AFB1, B[a]P, Glu-P-1, NQNO, and Trp-P-1. We found that both extracts have the strongest antimutagenic activity against Trp-P-1, followed by Glu-P-1, B[a]P, AFB1, and finally NQNO. In addition, the antimutagenicity of the extracts was produced in a concentration-dependent manner. At a concentration of 200 ppm, both extracts showed the highest inhibitory action. However, the linear correlation indicated that concentration-activity relationship was not significant (p > 0.05). In addition, extracts showed less antimutagenicity after heat treatment (p < 0.05). This suggests that the antimutagenicity of the extracts is heat-labile. The ethanol extract from mycelium or fruiting body had better antimutagenic effects than did the water extract (p < 0.05). Also, the extract from the fruiting body had better antimutagenic effects than did that from the mycelium. |
本系統中英文摘要資訊取自各篇刊載內容。