頁籤選單縮合
題名 | 盤克夏豬造骨蛋白基因啟動子區域之TG重複區定序=Nucleotide Sequencing of TG Repeats in the Promoter Region of Osteopontin Gene in Berkshire Pigs |
---|---|
作者 | 廖仁寶; 吳明哲; 張秀鑾; | 書刊名 | 中華農學會報 |
卷期 | 2:3 2001.06[民90.06] |
頁次 | 頁217-225 |
分類號 | 437.22 |
關鍵詞 | 豬; 造骨蛋白; 核苷酸定序; Pig; Osteopontin; Nucleotide sequencing; |
語文 | 中文(Chinese) |
中文摘要 | 造骨蛋白(OPN)基因啟動子區域含有TG序列重複區,本研究以PCR分別增幅七頭盤克夏豬的核內DNA,將其PCR產物接合至載體上,再轉形至大腸桿菌中。七頭豬的選殖株中,再各挑選4、6、5、4、6、3、和5個偈色菌落,培養後萃取其重組質體,再進行插入部分定序步驟。比較33個選殖株的插入部分序列,除TG重複數有不同外,其他部分皆相同。插入部分定序結果為5’CCAATCCTATTCACGAAAAAGCTAGTTAATGACATTGTACATAAGTAATGTTTTAACTGCAGAGGGCG[TG]NTTGTAACCACAAAACCAGAGGGGGAAGTGTGGGAGCAAGTGGGTTG3’(首尾兩端黑粗字體字為正反向引子位置)。同時,檢測62頭盤克夏種豬造骨蛋白基因型,在12頭公豬中,所發現的四種交替基因(頻率)分別為[TG]13 (0.08)、[TG]23 (0.13)、[TG]24 (0.13)、[TG]24 (0.63)、[TG]25 (0.17),共組成五種基因型。而在50頭母豬中,所發現的五種交替基因(頻率)分別為[TG]8 (0.03)、[TG]13 (0.15)、[TG]23 (0.12)、[TG]24 (0.65)、[TG]25 (0.05),共組成九種基因型。進一步分析種母豬具不同OPN交替基因對基產仔性能之影響,結果顯示:具[TG]13交替基因之盤克夏種母豬,其所分娩活仔數顯著地較未具該交替基因者為差。 |
英文摘要 | Porcine osteopontin (OPN) gene has the promoter region containing TG repeats. This study used seven DNA samples for Berkshire pigs for producing fragments by PCR. Then those PCR products were inserted into a commercial vector that the recombinant plasmid was transformed into E. coli. There were 4,6,5,4,6,3, and 5 colonies from those seven transformed, respectively, for purification and sequencing of their inserted DNA fragments. Sequence comparison was made on all 33 colonies, and the only difference was found on the number of TG repeats. Sequencing results revealed that the sequence of the promoter region of OPN gene was 5’CCAATCCTATTCACGAAAAAGCTAGTTAATGACATTGTAAGTAATGTTTTAACTGCAGAGGGCG[TG]NTTGTAACCACAAAACCAGAGGGGGAAGTGTGGGAGCAAGTGGGTTG3’ (The regions of the forward and reverse primers of PCR are presented in bold italics.). In the meantime, we identified the opn genotype of 62 stocks pigs. Among 12 sires there were four alleles in terms of TG repeat making up 5 genotypes, and the four alleles (frequencies) were [TG]13(0.08), [TG]23(0.13), [TG]24(0.62) and [TG]25(0.17), respectively. Identically, among 50 dams, there were five alleles in terms of TG repeat making up 9genotypes, and the five alleles (frequencies) were [TG]8(0.03), [TG]13(0.015), [TG]23(0.12), [TG]24(0.65) and [TG]25(0.05), respectively. When we further analyzed the litter performance of sows with different OPN alleles, the results indicated that the number of alive piglets born from Berkshire sows with [TG]13 allele were significantly less then those without such an allele. |
本系統之摘要資訊系依該期刊論文摘要之資訊為主。