查詢結果分析
相關文獻
- Effects of Drugs on Cellular Proliferation in Cultured Iris Pigment Epithelial Cells and Retinal Pigment Epithelial Cells
- Flutamide-Induced Liver Injury: A Case Report
- Outcome of Graves' Disease after Antithyroid Drug Treatment in Taiwan
- 簡介呃逆的藥物治療
- Intrathecal Opioids Provide Satisfactory Conditions in Labor Analgesia and Spinal Anesthesia for Cesarean Section
- 臺灣地區藥物濫用社會成本推估初報
- In Vitro Effects of Various Neuropharmacological Agents on the Motility of Adult Clonorchis Sinensis
- 抗風濕藥物
- 簡介冠狀動脈支架(Stents)與其藥物治療
- Rharmacological Prevention of Relapse
頁籤選單縮合
題 名 | Effects of Drugs on Cellular Proliferation in Cultured Iris Pigment Epithelial Cells and Retinal Pigment Epithelial Cells=藥物對虹膜色素上皮細胞及視網膜上皮細胞增生的影響 |
---|---|
作 者 | 吳國揚; 洪秀貞; 王惠珠; 黃佳惠; 賴昱宏; | 書刊名 | The Kaohsiung Journal of Medical Sciences |
卷 期 | 17:2 2001.02[民90.02] |
頁 次 | 頁77-83 |
分類號 | 418.2323 |
關鍵詞 | 藥物; 虹膜色素上皮細胞; 視網膜上皮細胞增生; Iris pigment epithelial cells; Retinal pigment epithelial cells; Cell proliferation; |
語 文 | 英文(English) |
中文摘要 | 本實驗主要是探討不同種類的藥包括methionine-enkephalin, isoproterenol, dibutyryl cAMP, endothelin-1, dexamethasone及phorbol 12-myristate 13-acetate(PMA)對體外培養的豬眼球虹膜色素上皮細胞及視網膜色素上皮細胞 對3H-thymidine攝取量的影響,結果顯示兩種細胞經過體外培養後皆呈現表皮 狀的形態,兩種細胞內亦含有許多色素顆粒。虹膜色素上皮細胞經過endothelin-1 處理後,細胞對3H-thymidine攝取量增加到控制組的126﹪,但是isoproterenol 卻降低虹膜色素上皮細胞的攝取量只剩下控制組的83﹪,其它的藥例如 methionine-enkephalin, dibutyryl cAMP, dexamethasone及PMA都不影響虹膜色素 上皮細胞的增生。然而藥物對視網膜色素上皮細胞的增生卻有不同的影響,結果 顯示methionine-enkephalin及isoproterenol皆分別可增加攝取量到控制組的145 ﹪及146﹪,但是dibutyryl cAMP, dexamethasone及PMA卻會抑制視網膜色素上 皮細胞對3H-thymidine攝取量分別只剩下83﹪,73﹪及85﹪,而endothelin-1 對視網膜色素上皮細胞的增生卻沒有影響,由以上結果顯示雖然虹膜色素上皮細 胞及視網膜色素上皮細胞二者的形態類似,但是調節二種色素細胞的增生機轉可 能不相同。 |
英文摘要 | In this study, iris and retinal pigment epithelial cells were cultured from porcine and various drugs including methionine-enkephalin, isoproterenol, dibutyryl cAMP, endothelin-1, dexamethasone and phorbol 12-myristate 13-acetate (PMA) were used to investigate their effects on both cellular proliferation in cultured porcine iris and retinal pigment epithelial cells. Cellular proliferation was estimated with 3H-thymidine uptake. It is indicated that both pigment epithelial cells possess epithelial-like morphology and abundant pigment granules in cells obviously. Following the iris pigment epithelial cells being treated with endothelin-1, the 3H-thymidine uptake in the cells was increased to 126% as compared with the control. However, the cellular proliferation was decreased to 83% when the cells were treated with isoproterenol. In the case of methionine-enkephalin, dibutyryl cAMP, dexamethasone and phorbol 12-myristate 13-acetate (PMA), the thymidine uptake in the iris pigment epithelial cells was not affected by above drugs. In the retinal pigment epithelial cells, the 3H-thymidine uptakes were increased to 145% and 146% when the cells were incubated with methionine-enkephalin, and isoproterenol, respectively. In the presence of dibutyryl cAMP, dexamethasone and phorbol ester (PMA), the cellular proliferation was inhibited to 83%, 73% and 85% respectively. However, endothelin-1 did not affect the cellular proliferation in retinal pigment epithelial cells. These results show that the morphological shapes of iris pigment epithelial cells are similar to retinal pigment epithelial cells. However, the cellular proliferation in both cells may be regulated by distinct mechanisms. |
本系統中英文摘要資訊取自各篇刊載內容。