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題 名 | 激活方式對小鼠及家兔卵母細胞激活率及原核形成時間分佈之影響=Activation Rate and Time-Distribution of Pronuclear Formation in Mouse and Rabbit Oocytes |
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作 者 | 沈朋志; 鄭三寶; 劉炳燦; 陳建宇; 顏淑卿; | 書刊名 | 中國畜牧學會會誌 |
卷 期 | 27:4 1998.12[民87.12] |
頁 次 | 頁519-532 |
分類號 | 437.68 |
關鍵詞 | 小鼠; 家兔; 卵母細胞; 激活作用; 原核形成; Mouse; Rabbit; Oocyte; Activation; Pronuclear formation; |
語 文 | 中文(Chinese) |
中文摘要 | 本研究旨在觀測取卵時機與激活處理方式對小鼠或家兔卵母細胞激活率及其原 核形成時間之分佈。結果顯示,早期(hCG注射後10~11小時)所取得之小鼠卵母細胞僅施以 4次電激處理之激活率明顯較晚期(hCG注射後14~16小時)者為低(74.1% vs 97.1%,P< 0.06);而早期取得之卵母細胞經4次電激後,再置入含10μg/ml亞胺環已酮( cycloheximide,CHX)之培養液中進行激活培養後之激活率明顯被提昇(95.6%)。惟不論 早或晚期取得之卵母細胞,經先後置入含7%酒精(ethanol)和10μg/ml CHX之激活液中進 行激活培養,所獲得之激活率相近(90.9%和91.4%,P>0.06);但其於激活開始迄第20 小時之各觀察時段所累積之原核形成率,均以晚期所取得之小鼠卵母細胞略優於早期所取 得者(第 2小時:6.9% vs 2.3%;第 3小時:13.8% vs 11.4%:第4小時:50%/5. 25%:第5小時:67.2% vs 52.3%;第10小時:89.7 vs 77.3%:第20小時:91.4% vs 90.9%)。若早期所取得之小鼠卵母細胞經1~4次之電激後,再置入含CHX之激活液中進行 激活培養可獲得100%之激活率,於處理開始後5小時,其原核形成率達97.6%~100%。在 hCG注射14~15小時後所取得之兔卵母細胞經歷1、3或6次之電激處理後,再置入含CHX之 激 活液中進行激活培養之激活率隨電激次數之增加而提昇(30.8%vs 76.9%,90.0%,P< 0.00l)。惟經6次電激後,再置入含CHX激活液中之激活處理並非必要,於處理開始後9小 時,約76.0%之原核形成。 |
英文摘要 | The efficiency of nuclear transplantation depends mainly on the enucleated rate, activation of recipient oocyte, and synchrony of cell cycle stage between nucleus and cytoplasm. The purpose of this study was to observe the effects of the collection time and activation methods on the activation rate and the time-distribution of pronuclear formation in mouse and rabbit oocytes. Results indicated that after stimulation by four electric pulses, mouse oocytes collected in the earlier period (10-11 hr post hCG injection) had a lower activation rate than those collected in later period (14-15 hr post hCG injection) (74.1% vs. 97.1%). Moreover, the activation rate of the oocyte collected in the earlier period was enhanced after being cultured in cycloheximide (CHX) followed the four-times electric stimuli (95.6%). There was no difference in the activation rate between mouse oocytes collected in the earlier and later period when the treatment of 7% ethanol with 10 U g/ml CHX was used for activation (90.9% vs. 91.4%). However, the proportion of the pronuclear formation was higher for the mouse oocytes collected in the earlier period when compared to those collected in the later period. Mouse oocytes collected in the earlier period could be totally activated (100%) when they were treated with CHX directly after electrical stimulation with one to four electrical pulses, with the majority (> 97.5%) being activated within five hours after treatments. Rabbit oocytes collected at 14-15 hr after hCG injection were subjected to activation by electrical stimulation with one, three or six pulses and CHX treatment. Activation rate was enhanced along with the number of pulses. The CHX treatment did not significantly improve the activation when rabbit oocytes were previously stimulated electrically by more than 6 pulses. Nine hours after activation the pronuclear formation rate was 75%. It was concludede that the oocyte could be activated by one electrical pulse followed by being cultured in medium containing 10 m g/ ml CHX in the mouse, while six electrical pulses were needed in the rabbit. |
本系統中英文摘要資訊取自各篇刊載內容。