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題名 | Inhibitory Characteristics of Methamidophos on House fly Head Acetylcholinesterase=達馬松對家蠅乙醯膽鹼酯酶抑制特質之研究 |
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作者 | 鄭允; 高靜華; 黃毓斌; 曾佳琳; | 書刊名 | 中華農業研究 |
卷期 | 49:4 2000.12[民89.12] |
頁次 | 頁89-98 |
分類號 | 433.85 |
關鍵詞 | 家蠅乙醯膽鹼酯酶; 達馬松; 酵素穩定性; 回收率; 敏感度; Housefly head acetylcholinesterase; Methamidophos; Enzyme stability; Incubation time; Recovery; |
語文 | 英文(English) |
中文摘要 | 早期曾利用農業試驗所飼養家蠅所純化之乙醯膽鹼酯?,測試其對達馬松作用3分鐘之敏感度,以25%抑制率為基準時之敏感度甚低。由於達馬松具有高水溶性以及作用於乙醯膽鹼酯?時反應較慢之特性,本研究仍著力於修正以乙醯膽鹼酯?檢測達馬松之方法,期能提高兩者間之敏感度。因乙醯膽鹼酯?可在室溫下反應30分鐘,而其活性仍保持不變,故與達馬松作用時經由不同反應時間(3~30分鐘)及不同濃度(0.3~4.6ppm之間)進行比較測試。結果顯示,作用5分鐘時,需4ppm達馬松才可達到36%抑制,但反應時間增長為30分鐘時,僅須0.6ppm達馬松即可達37%之抑制,檢測敏感度可提高近8倍。此外,檢測達馬松在葉片上殘留時,無需使用甲醇或乙醇萃取,改為直接以緩衝液萃取,結果甚為理想。經由抑制率之估算,緩衝液之萃取回收率幾達100%,可檢測之達馬松殘留量以植物體重量估算,約為2~4ppm。此一敏感度與容許量之範圍已甚為接近,而使乙醯膽鹼酯?用於檢測達馬松殘毒之應用性大幅提昇。 |
英文摘要 | The acetylcholinesterase (AChE) purified from housefly (Musca domestica L.) head of a TARI's colony has been tested for its sensitivity to methamidophos, a phosphoramidate that is highly water-soluble but with low sensitivity to AChE. The reaction of methamidophos to AChE was analyzed in vitro in several insecticide concentrations (0.3, 0.6, 1.2, 2.3 and 4.6ppm) at different incubation times. In general, the activity of AChE remains unchanged in room temperature for 30 min, and all the results confirm that action of methamidophos to AChE is slow but stable. By increasing the incubation time, strong inhibition can be resulted from low concentration of methamidophos. For example, 0.6ppm results in 37% inhibition in 30 min, while 4.6ppm only cause 36% inhibition in the 5 min treatment i.e., the 25 min difference in incubation time can cause 8-fold increase in insecticide sensitivity. By utilizing this character, the housefly head AChE can be used to detect the methamidophos residue in plant material simply by increasing the incubation time. In the actual measurement, almost 100% methamidophos residue was recovered by phosphate buffer extraction instead of methanol/ethanol extraction, and the sensitivity of AChE to methamidophos residue can extend to 2-4ppm level when testintg the marketable size cabbage. We conclude that by modifying the testing procedures, the hously head AChE is feasible for monitoring methamidophos residue on plant material. |
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